Probe:	Neph3 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: mesonephros	Present		1G
TS15: otocyst	Present		1G
TS15: pancreas primordium	Present		1G
TS15: future hindbrain	Present	Regionally restricted	1G	Expressed in a small area at the bottom of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	1H	Expressed in the ventral region.
TS17: hindbrain	Present	Regionally restricted	1H	Expressed in parts of the rhombencephalon and the teloceles.
TS17: mesonephros	Present		1H
TS17: otocyst	Present		1H
TS17: pancreas primordium	Present		1H

Structure	Level	Pattern	Image	Note
TS19: cerebellum primordium	Present		1I
TS19: neural tube	Present		1I
TS19: metanephros	Absent		1I
TS19: otocyst	Absent		1I
TS19: pancreas primordium	Absent		1I

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Strong	Regionally restricted	4A	Expressed at the bottom and the roof of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	4B	Expressed at the bottom and the roof of the fourth ventricle, and widened slightly in the rostro-occipital axis.

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present	Regionally restricted	4C	Expressed at the bottom and the roof of the fourth ventricle, and widened slightly in the rostro-occipital axis.

Structure	Level	Pattern	Image	Note
TS17: rhombomere 2	Present		4D	Expressed in a thin layer of cells delineating the border of the single ependymal cell layer comprising the roof plate and the alar plate.
TS17: rhombomere 3	Present		4D	Expressed in a thin layer of cells delineating the border of the single ependymal cell layer comprising the roof plate and the alar plate.
TS17: rhombomere 7	Present		4D	Expressed in a thin layer of cells delineating the border of the single ependymal cell layer comprising the roof plate and the alar plate.
TS17: neural tube	Present		4D

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present		4E
TS20: pons	Present		4E , 4F	Expressed in the pontal flexure extending processes towards the floor plates of the pertaining rhombomeres, while their cell bodies appeared to be ventricularly localized.
TS20: cerebellum primordium	Present		4H , 4E	Expressed in internal germ layer.

Structure	Level	Pattern	Image	Note
TS24: nasal epithelium	Present	Regionally restricted	4G	Expressed in the roof of the nasal cavity.