Probe:	Neph2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: dermomyotome	Present		1D	Expressed in dorsal somites, limited in the rostral region.
TS15: 2nd branchial arch	Present		1D
TS15: 3rd branchial arch	Present		1D
TS15: Rathke's pouch	Present		1D
TS15: mesonephros	Present		1D
TS15: otocyst	Present		1D

Structure	Level	Pattern	Image	Note
TS17: 2nd branchial arch	Present		1E
TS17: 3rd branchial arch	Present		1E
TS17: Rathke's pouch	Present		1E
TS17: otocyst	Present		1E
TS17: dermomyotome	Present		1E	Expressed in dorsal somites, limited in the rostral region.
TS17: mesonephros	Ambiguous		1E	The identity of this domain was unclear.

Structure	Level	Pattern	Image	Note
TS19: dermomyotome	Present		1F	Expression extended to the caudal somites.
TS19: Rathke's pouch	Present		1F
TS19: footplate apical ectodermal ridge	Present		1F
TS19: forelimb bud mesenchyme	Present		1F
TS19: handplate apical ectodermal ridge	Present		1F
TS19: hindlimb bud mesenchyme	Present		1F
TS19: metencephalon basal plate	Present	Regionally restricted	1F	Expressed in pons.
TS19: hindbrain	Present	Regionally restricted	1F	Expressed in medulla oblongata.
TS19: axial skeleton	Present	Regionally restricted	1F	Expressed in the primitive intervertebral discs.

Structure	Level	Pattern	Image	Note
TS19: footplate apical ectodermal ridge	Present		3A
TS19: forelimb bud mesenchyme	Present		3A
TS19: handplate apical ectodermal ridge	Present		3A
TS19: hindlimb bud mesenchyme	Present		3A

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		3B
TS17: forelimb bud mesenchyme	Absent		3B

Structure	Level	Pattern	Image	Note
TS24: neocortex	Present		3D , 3C	Expressed in superficial layers.
TS24: diencephalon	Present		3C , 3E
TS24: pretectum	Present		3E , 3C
TS24: thalamus	Present	Regionally restricted	3C , 3E	Expressed in the intermediate tier and a small band of cells bordering the rostral part of the dorsal tier.
TS24: medulla oblongata	Strong		3G , 3C
TS24: hindbrain	Strong	Regionally restricted	3F , 3G , 3C	Expressed as an oblong area extending caudally into the spinal cord, and in the basal plates of rhombomeres at the rostral part of the ponto-medullary transition zone.
TS24: hypothalamus	Present	Regionally restricted	3F , 3C	Expressed in the peduncular hypothalamus.