Runx1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:316418 Azzoni E, et al., The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition. Cell Rep. 2021 Dec 14;37(11):110103
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7276241
Gene symbol:	Runx1
Gene name:	runt related transcription factor 1

Antibody:	anti-RUNX1/AML1 + RUNX2 + RUNX3 (EPR3099; ab92336)
Detection system:	Secondary antibody

Results

1A CD31/Runx1 +/+ (left) ©

1A CD31/Runx1 +/+ (right) ©

Specimen 1A +/+ Runx1: embryonic day 8.25 (more

)
Note: 4-6 somite pairs. Double labeled: green - Runx1; white - CD31 (Pecam1 assay MGI:7276240).

Structure	Level	Pattern	Image	Note
TS12: dorsal aorta	Present	Single cells	1A CD31/Runx1 +/+ (right) , 1A CD31/Runx1 +/+ (left)	Expression in a few cells of posterior paired dorsal aortae.
TS12: vitelline artery	Present		1A CD31/Runx1 +/+ (left) , 1A CD31/Runx1 +/+ (right)

1A CD31/Runx1 -/- (left) ©

1A CD31/Runx1 -/- (right) ©

Specimen 1A -/- Runx1: embryonic day 8.25; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 4-6 somite pairs. Double labeled: green - Runx1; white - CD31 (Pecam1 assay MGI:7276240).

Structure	Level	Pattern	Image	Note
TS12: dorsal aorta	Present	Single cells	1A CD31/Runx1 -/- (left) , 1A CD31/Runx1 -/- (right)	Expression similar to that of wild type embryos.
TS12: vitelline artery	Present		1A CD31/Runx1 -/- (left) , 1A CD31/Runx1 -/- (right)

1B +/+ (left) ©

1B +/+ (right) ©

Specimen 1B +/+ Runx1: embryonic day 9.5 (more

)
Note: 22-26 somite pairs. Double labeled: green - Runx1; white - VE-Cad (Cdh5 assay MGI:7276242).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present	Regionally restricted	1B +/+ (left) , 1B +/+ (right)	Expression extended along dorsal aorta in aorta-gonad-mesonephros (AGM) region.
TS15: umbilical artery	Present	Regionally restricted	1B +/+ (left) , 1B +/+ (right)	Expression in proximal parts of vitelline and umbilical arteries.
TS15: vitelline artery	Present	Regionally restricted	1B +/+ (left) , 1B +/+ (right)	Expression in proximal parts of vitelline and umbilical arteries.

1B -/- (left) ©

1B -/- (right) ©

Specimen 1B -/- Runx1: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 22-26 somite pairs. Double labeled: green - Runx1; white - VE-Cad (Cdh5 assay MGI:7276242).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present	Regionally restricted	1B -/- (left) , 1B -/- (right)	Expression extended along dorsal aorta in aorta-gonad-mesonephros (AGM) region.
TS15: umbilical artery	Present	Regionally restricted	1B -/- (right) , 1B -/- (left)	Expression in proximal parts of vitelline and umbilical arteries.
TS15: vitelline artery	Present	Regionally restricted	1B -/- (right) , 1B -/- (left)	Expression in proximal parts of vitelline and umbilical arteries.

2A VE-Cad/CD43/Runx1 +/+ (left) ©

Specimen 2A +/+ (left) Runx1: embryonic day 9.5 (more

)
Note: 21-26 somite pairs. Triple labeled: green - Runx1; white - VE-Cad (Cdh5 assay MGI:7276242); red - CD43 (Spn assay MGI:7276244).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta endothelium	Present	Regionally restricted	2A VE-Cad/CD43/Runx1 +/+ (left)	Expression in hemogenic endothelium.

2A VE-Cad/CD43/runx1 -/- (left) ©

Specimen 2A -/- (left) Runx1: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 21-26 somite pairs. Triple labeled: green - Runx1; white - VE-Cad (Cdh5 assay MGI:7276242); red - CD43 (Spn assay MGI:7276244).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta endothelium	Present	Regionally restricted	2A VE-Cad/CD43/runx1 -/- (left)	Expression in hemogenic endothelium.

2A CD31/CD41/Runx1 +/+ ©

Specimen 2A +/+ (middle left) Runx1: embryonic day 9.5 (more

)
Note: 21-26 somite pairs. Triple labeled: green - Runx1; white - CD31 (Pecam1 assay MGI:7276240); red - CD41 (Itga2b assay MGI:7276245).

Structure	Cell Type	Level	Pattern	Image	Note
TS15: dorsal aorta	hematopoietic cell	Present		2A CD31/CD41/Runx1 +/+

2A CD31/CD41/Runx1 -/- ©

Specimen 2A -/- (middle left) Runx1: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 21-26 somite pairs. Triple labeled: green - Runx1; white - CD31 (Pecam1 assay MGI:7276240); red - CD41 (Itga2b assay MGI:7276245).

Structure	Cell Type	Level	Pattern	Image	Note
TS15: dorsal aorta	hematopoietic cell	Present		2A CD31/CD41/Runx1 -/-

2A VE-Cad/CD43/Runx1 +/+ (right) ©

Specimen 2A +/+ (right) Runx1: embryonic day 9.5 (more

)
Note: 21-26 somite pairs. Triple labeled: green - Runx1; white - VE-Cad (Cdh5 assay MGI:7276242); red - CD43 (Spn assay MGI:7276244).

Structure	Cell Type	Level	Pattern	Image	Note
TS15: vitelline artery	hematopoietic cell	Present		2A VE-Cad/CD43/Runx1 +/+ (right)

2A VE-Cad/CD43/Runx1 -/- (right) ©

Specimen 2A -/- (right) Runx1: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 21-26 somite pairs. Triple labeled: green - Runx1; white - VE-Cad (Cdh5 assay MGI:7276242); red - CD43 (Spn assay MGI:7276244).

Structure	Level	Pattern	Image	Note
TS15: vitelline artery	Present	Regionally restricted	2A VE-Cad/CD43/Runx1 -/- (right)	Expression in hemogenic endothelium.

2F Runx1 +/+ ©

2F VE-Cad/Jag1/Runx1 +/+ ©

Specimen 2F +/+ Runx1: embryonic day 9.5 (more

)
Note: 23-25 somite pairs. Triple labeled: green - Runx1; white - VE-Cad (Cdh5 assay MGI:7276242); red - Jag1 (assay MGI:7276246).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		2F VE-Cad/Jag1/Runx1 +/+ , 2F Runx1 +/+

2F Runx1 -/- ©

2F VE-Cad/Jag1/Runx1 -/- ©

Specimen 2F -/- Runx1: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 23-25 somite pairs. Triple labeled: green - Runx1; white - VE-Cad (Cdh5 assay MGI:7276242); red - Jag1 (assay MGI:7276246).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		2F Runx1 -/- , 2F VE-Cad/Jag1/Runx1 -/-

3A +/+ (left) ©

Specimen 3A +/+ Runx1: embryonic day 9.5 (more

)
Note: 22-25 somite pairs. Triple labeled: green - Runx1; white - CD31 (Pecam1 assay MGI:7276240); red - alpha-SMA (Acta2 assay MGI:7276247).

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		3A +/+ (left)

3A -/- (left) ©

Specimen 3A -/- Runx1: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 22-25 somite pairs. Triple labeled: green - Runx1; white - CD31 (Pecam1 assay MGI:7276240); red - alpha-SMA (Acta2 assay MGI:7276247).

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		3A -/- (left)

3A +/+ (middle) ©

Specimen 3A +/+ slice Runx1: embryonic day 9.5 (more

)
Note: 22-25 somite pairs. Triple labeled: green - Runx1; white - CD31 (Pecam1 assay MGI:7276240); red - alpha-SMA (Acta2 assay MGI:7276247).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		3A +/+ (middle)
TS15: vitelline artery	Present		3A +/+ (middle)

Specimen 3A -/- slice Runx1: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 22-25 somite pairs. Triple labeled: green - Runx1; white - CD31 (Pecam1 assay MGI:7276240); red - alpha-SMA (Acta2 assay MGI:7276247).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		3A -/- (middle)
TS15: vitelline artery	Present		3A -/- (middle)

3E +/+ ©

Specimen 3E +/+ Runx1: embryonic day 9.5 (more

)
Note: 21-24 somite pairs. Triple labeled: green - Runx1; white - CD31 (Pecam1 assay MGI:7276240); red - F4/80 (Adgre1 assay MGI:7276248).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		3E +/+

3E -/- ©

Specimen 3E -/- Runx1: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 21-24 somite pairs. Triple labeled: green - Runx1; white - CD31 (Pecam1 assay MGI:7276240); red - F4/80 (Adgre1 assay MGI:7276248).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		3E -/-
TS15: vitelline artery	Present		3E -/-

Specimen S1D +/+ (upper) Runx1: embryonic day 9.5 (more

)
Note: 21-26 somite pairs. Triple labeled: green - Runx1; white - CD31 (Pecam1 assay MGI:7276240); red - c-Kit (Kit assay MGI:7276248).

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present		S1D CD31/c-Kit/Runx1 +/+

Specimen S1D -/- (upper) Runx1: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 21-26 somite pairs. Triple labeled: green - Runx1; white - CD31 (Pecam1 assay MGI:7276240); red - c-Kit (Kit assay MGI:7276248).

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present		S1D CD31/c-Kit/Runx1 -/-

Specimen S1D +/+ (lower) Runx1: embryonic day 9.5 (more

)
Note: 21-26 somite pairs. Triple labeled: green - Runx1; white - VE-Cad (Cdh5 assay MGI:7276242); red - CD43 (Spn assay MGI:7276244).

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present		S1D VE-Cad/CD43/Runx1 +/+

Specimen S1D -/- (lower) Runx1: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 21-26 somite pairs. Triple labeled: green - Runx1; white - VE-Cad (Cdh5 assay MGI:7276242); red - CD43 (Spn assay MGI:7276244).

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present		S1D VE-Cad/CD43/Runx1 -/-

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