Antibody:	anti-neurofilament clone 2H3
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present		3A
TS17: spinal nerve	Present		3A

Structure	Level	Pattern	Image	Note
TS17: cranial nerve	Present		3B	Development of the cranial and spinal nerve systems was disorganize compared with the wild-type.
TS17: spinal nerve	Present		3B	Development of the cranial and spinal nerve systems was disorganize compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present		3C	Projections from the mesencephalic neurons were fasciculated.

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present	Regionally restricted	3D	Axonal projection of the ophthalmic branch of the trigeminal nerve was aberrant. The ascending axons of the glossopharyngeal nerve (IX) were fused with those of the vagus nerve (X). Projections from the mesencephalic neurons were not fasciculated.

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present		3E

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present	Regionally restricted	3F	The hypoglossal nerve (XII) was poorly formed compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		6A	Neurons (Mes5) in the mesencephalic alar plate projected their axons ventrally first and then caudally to the trigeminal ganglia.
TS15: facio-acoustic preganglion complex	Present		6A
TS15: future hindbrain	Present		6A
TS15: glossopharyngeal preganglion	Present		6A
TS15: trigeminal preganglion	Present		6A
TS15: vagus preganglion	Present		6A

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		6B	Axonal projections from the mesencephalic neurons were aberrant and not properly fasciculated, compared with the wild-type.
TS15: facio-acoustic preganglion complex	Present		6B
TS15: future hindbrain	Present		6B
TS15: glossopharyngeal preganglion	Present		6B
TS15: trigeminal preganglion	Present		6B
TS15: vagus preganglion	Present		6B

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		6C	Neurons (Mes5) in the mesencephalic alar plate projected their axons ventrally first and then caudally to the trigeminal ganglia.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present		6D	Axonal projections from the mesencephalic neurons were aberrant and not properly fasciculated, compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present		6G	Neurons (Mes5) in the mesencephalic alar plate projected their axons ventrally first and then caudally to the trigeminal ganglia.
TS17: trigeminal ganglion	Present		6G
TS17: hindbrain	Present		6G	Axons are fasciculated and form a tract.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present		6H	Axonal projections from the mesencephalic neurons were aberrant and not properly fasciculated, compared with the wild-type.
TS17: midbrain	Present		6H	Axonal projections from the mesencephalic neurons were aberrant and not properly fasciculated, compared with the wild-type.
TS17: trigeminal ganglion	Present		6H

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present		6I	Neurons (Mes5) in the mesencephalic alar plate projected their axons ventrally first and then caudally to the trigeminal ganglia.
TS17: hindbrain	Present		6I	Axons are fasciculated and form a tract.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present		6J	Axonal projections from the mesencephalic neurons were aberrant and not properly fasciculated, compared with the wild-type.
TS17: midbrain	Present		6J	Axonal projections from the mesencephalic neurons were aberrant and not properly fasciculated, compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present		6K	Neurons (Mes5) in the mesencephalic alar plate projected their axons ventrally first and then caudally to the trigeminal ganglia.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Present		6L	Axonal projections from the mesencephalic neurons were aberrant and not properly fasciculated, compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present		7A
TS17: spinal nerve	Present		7A

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present	Regionally restricted	7B	Some segments lack the spinal nerves.
TS17: dorsal root ganglion	Present		7B

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present		7C	The ventral roots from the motor neurons and the dorsal roots from the dorsal root ganglion were formed.
TS17: dorsal root ganglion	Present		7C

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present	Regionally restricted	7F	The ventral roots were mostly lacking.

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present	Regionally restricted	7G	The ventral roots were aberrantly projecting.

Structure	Level	Pattern	Image	Note
TS17: spinal nerve	Present	Regionally restricted	7H	The ventral roots were poorly formed.