Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:316552
Radojevic V, et al., Expression and localization of somatostatin receptor types 3, 4 and 5 in the wild-type, SSTR1 and SSTR1/SSTR2 knockout mouse cochlea. Cell Tissue Res. 2014 Dec;358(3):717-27
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7264355
|
|
Gene symbol:
|
Sstr4
|
|
Gene name:
|
somatostatin receptor 4
|
|
Antibody:
|
anti-SSTR4
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor
|
|
|
Results
|
Specimen
1e:
embryonic day 14.0
(more  )
Note: Double labeled: red - Sstr4; green - Hes6 (assay MGI:7264353).
Specimen
1e:
(close  )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Sstr4; green - Hes6 (assay MGI:7264353).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlear duct epithelium |
Present |
Regionally restricted |
1e
|
Expression in thickened epithelial cells located in Kolliker's organ in cochlear duct.
|
Specimen
1f:
embryonic day 17.0
(more )
Note: Double labeled: red - Sstr4; green - Hes6 (assay MGI:7264353).
Specimen
1f:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Sstr4; green - Hes6 (assay MGI:7264353).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: cochlear duct epithelium |
auditory hair cell |
Present |
Regionally restricted |
1f
|
Expression in region of hair cells and supporting cells along length of cochlear duct. Expression co-localized with that of Hes6.
|
| TS25: cochlear duct epithelium |
supporting cell of cochlea |
Present |
Regionally restricted |
1f
|
Expression in region of hair cells and supporting cells along length of cochlear duct. Expression co-localized with that of Hes6.
|
Specimen
3c:
postnatal adult
(more )
Specimen
3c:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
3d:
postnatal adult
(more )
Specimen
3d:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
supporting cell of cochlea |
Strong |
Regionally restricted |
3d
|
Prominent expression in supporting cells of outer hair cells.
|
| TS28: cochlea |
cochlear outer hair cell |
Present |
|
3d
|
|
| TS28: cochlea |
cochlear inner hair cell |
Strong |
Regionally restricted |
3d
|
Strong expression in apical part of inner hair cells.
|
Specimen
5d:
postnatal adult
(more )
Note: Double labeled: red - Sstr4; green - Myo7a (assay MGI:7264358).
Specimen
5d:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Sstr4; green - Myo7a (assay MGI:7264358).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
cochlear inner hair cell |
Present |
|
5d
|
|
| TS28: cochlea |
cochlear outer hair cell |
Present |
|
5d
|
|
Specimen
5e:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6
|
|
Mutant Allele(s):
|
Sstr1tm1Dric/Sstr1tm1Dric
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Sstr4; green - Myo7a (assay MGI:7264358).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
cochlear inner hair cell |
Present |
|
5e
|
No difference between wild type and Sstr1 knockout mice.
|
| TS28: cochlea |
cochlear outer hair cell |
Present |
|
5e
|
No difference between wild type and Sstr1 knockout mice.
|
Specimen
5f:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * 129S7/SvEvBrd * C57BL/6
|
|
Mutant Allele(s):
|
Sstr1tm1Dric/Sstr1tm1Dric
Sstr2tm1Rgs/Sstr2tm1Rgs
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Sstr4; green - Myo7a (assay MGI:7264358).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
cochlear inner hair cell |
Present |
Regionally restricted |
5f
|
Expression localized to apical part of hair cells.
|
| TS28: cochlea |
cochlear outer hair cell |
Present |
Regionally restricted |
5f
|
Expression localized to apical part of hair cells.
|
|
Analysis Tools