Gene Expression Data
Assay Details
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Assay
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Reference:
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J:316552
Radojevic V, et al., Expression and localization of somatostatin receptor types 3, 4 and 5 in the wild-type, SSTR1 and SSTR1/SSTR2 knockout mouse cochlea. Cell Tissue Res. 2014 Dec;358(3):717-27
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7264354
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Gene symbol:
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Sstr3
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Gene name:
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somatostatin receptor 3
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Antibody:
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anti-SSTR3
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
1b:
embryonic day 14.0
(more  )
Note: Double labeled: red - Sstr3; green - Hes6 (assay MGI:7264353).
Specimen
1b:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Sstr3; green - Hes6 (assay MGI:7264353).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlear duct epithelium |
Present |
Regionally restricted |
1b
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Expression in thickened epithelial cells located in Kolliker's organ in cochlear duct.
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Specimen
1c:
embryonic day 17.0
(more )
Note: Double labeled: red - Sstr3; green - Hes6 (assay MGI:7264353).
Specimen
1c:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Sstr3; green - Hes6 (assay MGI:7264353).
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Specimen
3a:
postnatal adult
(more )
Specimen
3a:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3b:
postnatal adult
(more )
Specimen
3b:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
cochlear inner hair cell |
Strong |
Regionally restricted |
3b
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Prominent expression in apical part of inner hair cells and outer hair cells
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| TS28: cochlea |
cochlear outer hair cell |
Strong |
Regionally restricted |
3b
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Prominent expression in apical part of inner hair cells and outer hair cells
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| TS28: organ of Corti |
pillar cell |
Strong |
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3b
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| TS28: cochlear ganglion |
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Strong |
Regionally restricted |
3b
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Expression in spiral ganglion cell bodies.
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Specimen
5a:
postnatal adult
(more )
Note: Double labeled: red - Sstr3; green - Myo7a (assay MGI:7264358).
Specimen
5a:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Sstr3; green - Myo7a (assay MGI:7264358).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
cochlear inner hair cell |
Present |
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5a
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| TS28: cochlea |
cochlear outer hair cell |
Present |
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5a
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| TS28: cochlea |
supporting cell of cochlea |
Present |
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5a
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Specimen
5b:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Sstr1tm1Dric/Sstr1tm1Dric
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Sstr3; green - Myo7a (assay MGI:7264358).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
cochlear inner hair cell |
Present |
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5b
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No difference between wild type and Sstr1 knockout mice.
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| TS28: cochlea |
cochlear outer hair cell |
Present |
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5b
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No difference between wild type and Sstr1 knockout mice.
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| TS28: cochlea |
supporting cell of cochlea |
Present |
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5b
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No difference between wild type and Sstr1 knockout mice.
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Specimen
5c:
(close )
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Genetic Background:
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involves: 129S4/SvJae * 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sstr1tm1Dric/Sstr1tm1Dric
Sstr2tm1Rgs/Sstr2tm1Rgs
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Sstr3; green - Myo7a (assay MGI:7264358).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
cochlear inner hair cell |
Weak |
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5c
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Expression restricted to inner hair cells and outer hair cells. Expression reduced compared to wild type.
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| TS28: cochlea |
cochlear outer hair cell |
Weak |
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5c
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Expression restricted to inner hair cells and outer hair cells. Expression reduced compared to wild type.
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| TS28: organ of Corti |
Deiter's cell |
Absent |
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5c
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| TS28: organ of Corti |
pillar cell |
Absent |
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5c
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Analysis Tools