Reporter:	lacZ
	Direct Detection
Assay notes:	Fixative of adult brains, developing brains, spinal cord, and optic nerves was 1% paraformaldehyde followed by 0.5% glutaraldehyde. Adult cochlea were fixed in 4% paraformaldehyde and 0.2% glutaraldehyde. Retinas and olfactory system were fixed in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS28: amygdaloid nucleus	Present
TS28: anterior cortical amygdaloid nucleus	Present
TS28: anterior dorsal thalamic nucleus	Present
TS28: anterior medial thalamic nucleus	Present
TS28: anterior tegmental nucleus	Present
TS28: anterior ventral thalamic nucleus	Present
TS28: caudate-putamen	Present
TS28: central amygdaloid nucleus	Present
TS28: central medial thalamic nucleus	Present
TS28: cerebral internal capsule	Present
TS28: cuneate nucleus	Present
TS28: dentate nucleus	Present
TS28: dorsal cochlear nucleus	Present
TS28: dorsal lateral geniculate nucleus	Present
TS28: fasciculus retroflexus	Present
TS28: fimbria hippocampus	Present
TS28: hippocampus CA1	Present
TS28: hippocampus CA2	Present
TS28: hypoglossal nucleus	Present
TS28: hypothalamus medial zone tuberal area	Present
TS28: indusium griseum	Present
TS28: insular cortex	Present
TS28: interpeduncular nucleus	Present
TS28: lateral habenular nucleus	Present
TS28: lateral mammillary nucleus	Present
TS28: lateral preoptic nucleus	Present
TS28: laterodorsal tegmental nucleus	Present
TS28: medial dorsal thalamic nucleus	Present
TS28: medial geniculate nucleus	Present
TS28: medial habenular nucleus	Present
TS28: medial mammillary nucleus	Present
TS28: medial preoptic nucleus	Present
TS28: median eminence	Present
TS28: nucleus of trapezoid body	Present
TS28: paraventricular thalamic nucleus	Present
TS28: piriform cortex	Present
TS28: posterior thalamic group	Present
TS28: primary somatosensory cortex	Present
TS28: solitary tract nucleus	Present
TS28: substantia nigra pars compacta	Present
TS28: subthalamic nucleus	Present
TS28: superior colliculus	Present
TS28: suprachiasmatic nucleus	Present
TS28: supramammillary nucleus	Present
TS28: trigeminal V principal sensory nucleus	Present
TS28: trigeminal ganglion	Present
TS28: ventral lateral geniculate nucleus	Present
TS28: ventral lateral thalamic nucleus	Present
TS28: ventral medial thalamic nucleus	Present
TS28: ventral posterior lateral thalamic nucleus	Present
TS28: ventral posterior medial thalamic nucleus	Present
TS28: ventromedial hypothalamic nucleus	Present
TS28: amygdalohippocampal area	Present	Regionally restricted		Expression is in the amygdalohippocampal area posterior medial.
TS28: thalamic nucleus	Present	Regionally restricted		Expression is in the xiphoid thalamic nucleus, submedius thalamic nucleus, posterior limitans thalamic nucleus, paracentral thalamic nucleus, nucleus ansa lenticularis, laterodorsal thalamic nucleus dorsomedia, laterodorsal thalamic nucleus ventrolateral and lateral posterior thalamic nucleus mediocaudal.
TS28: anterior pretectal nucleus	Present	Regionally restricted		Expression is in the anterior pretectal nucleus dorsal.
TS28: arcuate nucleus	Present	Regionally restricted		Expression is arcuate hypothalamic nucleus.
TS28: pallidum	Present	Regionally restricted		Expression is ventral.
TS28: inferior colliculus	Present	Regionally restricted		Expression is in the brachium inferior colliculus and external cortex inferior colliculus.
TS28: bed nucleus of stria terminalis	Present	Regionally restricted		Expression is in the bed nucleus of the stria terminalis lateral division posterior, ventral and medial.
TS28: pons	Present	Regionally restricted		Expression is in the central gray pons and in transverse fibers.
TS28: fornix	Present	Regionally restricted		Expression is dorsal.
TS28: orbital area	Present	Regionally restricted		Expression is in the dorsolateral orbital cortex.
TS28: periaqueductal grey	Present	Regionally restricted		Expression is in the dorsal lateral and dorsomedial periaqueductal gray area.
TS28: pontine nucleus	Present	Regionally restricted		Expression is in the dorsomedial pontine nucleus.
TS28: trigeminal V spinal sensory nucleus	Present	Regionally restricted		Expression is in the dorsomedial spinal trigeminal nucleus.
TS28: optic tract	Present	Regionally restricted		Expression is in the dorsal terminal nucleus of the accessory optic tract and lateral terminal nucleus accessory optic tract.
TS28: cochlear nucleus	Present	Regionally restricted		Expression is in the granular layer cochlear nucleus.
TS28: nucleus of diagonal band	Present	Regionally restricted		Expression is in the nucleus horizontal limb diagonal band.
TS28: anterior commissure	Present	Regionally restricted		Expression is in the interstriatal nucleus posterior limb-anterior commissure lateral and medial.
TS28: parabrachial nucleus	Present	Regionally restricted		Expression is central and dorsal.
TS28: lateral septal nucleus	Present	Regionally restricted		Expression is dorsal and intermediate.
TS28: inferior olivary nucleus	Present	Regionally restricted		Expression is in the lateral superior olive.
TS28: neocortex region	Present	Regionally restricted		Expression is in the medial orbital cortex.
TS28: preoptic area	Present	Regionally restricted		Expression is medial.
TS28: vestibular nucleus	Present	Regionally restricted		Expression is in the medial vestibular nucleus mediocaudal and paravicel.
TS28: paraventricular hypothalamic nucleus	Present	Regionally restricted		Expression is in the paraventricular hypothalamic anterior parvicellular and ventral.
TS28: brain nucleus	Present	Regionally restricted		Expression is in the precommissural nucleus.
TS28: nerve fiber tract	Present	Regionally restricted		Expression is in the pyramidal tract.
TS28: raphe nucleus	Present	Regionally restricted		Expression is in the raphe capsule.
TS28: periolivary nucleus	Present	Regionally restricted		Expression is in the rostral periolivary region.
TS28: midbrain tegmentum	Present	Regionally restricted		Expression is in the paralemniscal nucleus and in the visual tegmental relay zone and in the retrorubral field.
TS28: hypothalamic nucleus	Present	Regionally restricted		Expression is in the terete hypothalamus.
TS28: ventral cochlear nucleus	Present	Regionally restricted		Expression is posterior.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2A

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2B

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2C

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2D

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2E

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2F

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2G

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2H

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2I

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2J

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2K

Structure	Level	Pattern	Image	Note
TS28: brain	Present		2L

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Regionally restricted	3A	Expression is in the dorsolateral orbital cortex.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong		3B
TS28: hippocampus	Strong		3B

Structure	Level	Pattern	Image	Note
TS28: lateral septal nucleus	Present		3C

Structure	Level	Pattern	Image	Note
TS28: indusium griseum	Present		3D

Structure	Level	Pattern	Image	Note
TS28: medial habenular nucleus	Strong		3E

Structure	Level	Pattern	Image	Note
TS28: brain nucleus	Present	Regionally restricted	3F	Expression is in the interstriatal nucleus of the posterior limb of the anterior commissure, lateral part.

Structure	Level	Pattern	Image	Note
TS28: substantia nigra	Present		3G

Structure	Level	Pattern	Image	Note
TS28: interpeduncular nucleus	Strong		3H
TS28: pontine nucleus	Strong		3H

Structure	Level	Pattern	Image	Note
TS28: medial mammillary nucleus	Strong		3I

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	4A E18	Expression is at the surface.
TS26: brainstem	Strong		4A E18
TS26: hypothalamus	Strong		4A E18
TS26: thalamus	Strong		4A E18

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	4A P0	Expression is at the surface.
TS27: brainstem	Strong		4A P0
TS27: hypothalamus	Strong		4A P0
TS27: inferior colliculus	Strong		4A P0
TS27: superior colliculus	Strong		4A P0
TS27: thalamus	Strong		4A P0

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Present		4A P7	Expression is in many layers of the cortex.

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Present		4A P14
TS28: cortical layer I	Present		4A P14
TS28: cortical layer II	Present		4A P14
TS28: cortical layer III	Present		4A P14
TS28: cortical layer IV	Present		4A P14
TS28: cortical layer V	Present		4A P14
TS28: cortical layer VI	Present		4A P14
TS28: main olfactory bulb	Present		4A P14

Structure	Level	Pattern	Image	Note
TS28: cerebellum white matter	Present		4A Adult
TS28: cortical layer I	Present		4A Adult
TS28: cortical layer II	Present		4A Adult
TS28: cortical layer III	Present		4A Adult
TS28: cortical layer IV	Present		4A Adult
TS28: cortical layer V	Present		4A Adult
TS28: cortical layer VI	Present		4A Adult
TS28: main olfactory bulb	Present		4A Adult

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Present		4C E18	Expression started in the dentate gyrus at E18 and gradually with development moved to the CA1 and CA2 regions of the hippocampus.

Structure	Level	Pattern	Image	Note
TS27: dentate gyrus	Present	Regionally restricted	4C P0	Expression started in the dentate gyrus at E18 and gradually with development moved to the CA1/CA2 regions of the hippocampus.
TS27: hippocampus CA1	Present	Regionally restricted	4C P0	Expression started in the dentate gyrus at E18 and gradually with development moved to the CA1/CA2 regions of the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		4C P7
TS28: hippocampus CA1	Present		4C P7
TS28: hippocampus CA2	Present		4C P7

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Present		4C P14
TS28: hippocampus CA2	Present		4C P14
TS28: dentate gyrus	Weak		4C P14

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA1	Present		4C Adult
TS28: hippocampus CA2	Present		4C Adult
TS28: dentate gyrus	Weak		4C Adult

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex marginal zone	Present	Regionally restricted	4D E18	Expression is at the surface.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex marginal zone	Present	Regionally restricted	4D P0	Expression is at the surface.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	4D P7	Expression is in many layers of the cortex.

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Present		4D P14
TS28: cortical layer II	Present		4D P14
TS28: cortical layer III	Present		4D P14
TS28: cortical layer IV	Present		4D P14
TS28: cortical layer V	Present		4D P14
TS28: cortical layer VI	Present		4D P14

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Present		4D Adult
TS28: cortical layer II	Present		4D Adult
TS28: cortical layer III	Present		4D Adult
TS28: cortical layer IV	Present		4D Adult
TS28: cortical layer V	Present		4D Adult
TS28: cortical layer VI	Present		4D Adult

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	5A	Expression is in areas involved in sensory processing.
TS28: piriform cortex	Present	Regionally restricted	5A

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	5B	Expression is in areas involved in sensory processing.
TS28: medial geniculate nucleus	Present		5B

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	5C	Expression is in areas involved in sensory processing.
TS28: ventral posterior lateral thalamic nucleus	Present		5C
TS28: ventral posterior medial thalamic nucleus	Present		5C

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted	5D	Expression is in areas involved in sensory processing.
TS28: dorsal cochlear nucleus	Present		5D
TS28: solitary tract nucleus	Present		5D
TS28: trigeminal V spinal sensory nucleus	Present		5D

Structure	Level	Pattern	Image	Note
TS28: retina	Present		6A

Structure	Level	Pattern	Image	Note
TS28: optic nerve	Present		6B

Structure	Level	Pattern	Image	Note
TS28: lateral geniculate nucleus	Present		6C

Structure	Level	Pattern	Image	Note
TS28: central nervous system nerve	Present	Regionally restricted	6D	Expression is in the visual pathway leading from the retina to the superior colliculus.
TS28: retina	Present	Regionally restricted	6D	Expression is in the visual pathway leading from the retina to the superior colliculus.
TS28: superior colliculus	Present	Regionally restricted	6D	Expression is in the visual pathway leading from the retina to the superior colliculus.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: organ of Corti		Present	Regionally restricted	6F , 6E	Expression is in the inner spiral plexus and is absent in the inner or outer hair cells.
TS28: organ of Corti	cochlear inner hair cell	Absent		6F , 6E	Expression is in the inner spiral plexus and is absent in the inner or outer hair cells.
TS28: organ of Corti	cochlear outer hair cell	Absent		6F , 6E	Expression is in the inner spiral plexus and is absent in the inner or outer hair cells.
TS28: cochlear ganglion		Present		6E , 6F

Structure	Level	Pattern	Image	Note
TS28: dorsal cochlear nucleus	Present		6G

Structure	Level	Pattern	Image	Note
TS28: medial geniculate nucleus	Present		6H

Structure	Level	Pattern	Image	Note
TS23: hindlimb nerve	Present	Regionally restricted	6I	Expression is in nerves innervating the footpad.
TS23: metapodial pad	Present	Regionally restricted	6I	Expression is in nerves innervating the footpad.

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Present		6J

Structure	Level	Pattern	Image	Note
TS28: spinal cord dorsal horn	Present		6K

Structure	Level	Pattern	Image	Note
TS28: ventral posterior lateral thalamic nucleus	Present		6L

Structure	Level	Pattern	Image	Note
TS26: mystacial pad	Present	Regionally restricted	6M	Expression is in the innervation of the maxillary whiskers.

Structure	Level	Pattern	Image	Note
TS28: nerve	Present	Regionally restricted	6N	Expression is in the innervation of the tongue.
TS28: tongue	Present	Regionally restricted	6N	Expression is in the innervation of the tongue.

Structure	Level	Pattern	Image	Note
TS28: nerve	Present	Regionally restricted	6O	Expression is in axon bundles within the tongue.
TS28: tongue	Present	Regionally restricted	6O	Expression is in axon bundles within the tongue.

Structure	Level	Pattern	Image	Note
TS28: solitary tract nucleus	Present		6P

Structure	Level	Pattern	Image	Note
TS28: ventral posterior lateral thalamic nucleus	Present		6Q

Structure	Level	Pattern	Image	Note
TS28: olfactory nerve	Present		7B

Structure	Level	Pattern	Image	Note
TS28: olfactory nerve	Present		7C

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb external plexiform layer	Present		7D
TS28: main olfactory bulb glomerular layer	Present		7D
TS28: main olfactory bulb mitral cell layer	Present		7D

Structure	Level	Pattern	Image	Note
TS28: vomeronasal organ	Present	Regionally restricted	7E	Expression is in the basal vomeronasal sensory neurons.

Structure	Level	Pattern	Image	Note
TS28: vomeronasal organ	Present		7F	Expression includes the vomeronasal sensory neurons axons converging on the accessory olfactory bulb.
TS28: olfactory epithelium	Present		7F

Structure	Level	Pattern	Image	Note
TS28: accessory olfactory bulb	Strong	Regionally restricted	7G	Expression is posterior.

Structure	Level	Pattern	Image	Note
TS28: accessory olfactory bulb	Strong	Regionally restricted	7H	Expression is posterior with lower levels in the anterior part.

Structure	Level	Pattern	Image	Note
TS28: bed nucleus of stria terminalis	Present		7I
TS28: medial preoptic nucleus	Present		7I

Structure	Level	Pattern	Image	Note
TS28: medial thalamic group	Present		7J

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Present		7K

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S1A	The authors state that a comparison of the staining pattern from the homozygous and heterozygous brains showed differences in levels of expression but no gross differences in the regions that have expression.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S1B	The authors state that a comparison of the staining pattern from the homozygous and heterozygous brains showed differences in levels of expression but no gross differences in the regions that have expression.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S1C	The authors state that a comparison of the staining pattern from the homozygous and heterozygous brains showed differences in levels of expression but no gross differences in the regions that have expression.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S1D	The authors state that a comparison of the staining pattern from the homozygous and heterozygous brains showed differences in levels of expression but no gross differences in the regions that have expression.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S1E	The authors state that a comparison of the staining pattern from the homozygous and heterozygous brains showed differences in levels of expression but no gross differences in the regions that have expression.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S1F	The authors state that a comparison of the staining pattern from the homozygous and heterozygous brains showed differences in levels of expression but no gross differences in the regions that have expression.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S1G	The authors state that a comparison of the staining pattern from the homozygous and heterozygous brains showed differences in levels of expression but no gross differences in the regions that have expression.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S1H	The authors state that a comparison of the staining pattern from the homozygous and heterozygous brains showed differences in levels of expression but no gross differences in the regions that have expression.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S1I	The authors state that a comparison of the staining pattern from the homozygous and heterozygous brains showed differences in levels of expression but no gross differences in the regions that have expression.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S1J	The authors state that a comparison of the staining pattern from the homozygous and heterozygous brains showed differences in levels of expression but no gross differences in the regions that have expression.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S1K	The authors state that a comparison of the staining pattern from the homozygous and heterozygous brains showed differences in levels of expression but no gross differences in the regions that have expression.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		S1L	The authors state that a comparison of the staining pattern from the homozygous and heterozygous brains showed differences in levels of expression but no gross differences in the regions that have expression.