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Gene Expression Data
Assay Details
Assay
Reference: J:306253 Flemmer RT, et al., The Role of Mcl-1 in Embryonic Neural Precursor Cell Apoptosis. Front Cell Dev Biol. 2021;9:659531
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7260148
Gene symbol: Casp3
Gene name: caspase 3
Antibody: anti-caspase-3 (clone C92-605) (570863)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2B E9: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: prosencephalon Absent 2B E9 No evidence of apoptotic cells.
Specimen 2B' E9: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: prosencephalon Absent 2B' E9 No evidence of apoptotic cells.
Specimen 2B E10: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: future forebrain Trace 2B E10 Few to no apoptotic cells in control littermates
Specimen 2B' E10: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: future forebrain Trace 2B' E10 Few to no apoptotic cells in control littermates
Specimen 2B E11: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: forebrain Trace 2B E11 Few to no apoptotic cells in control littermates
Specimen 2B' E11: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: forebrain Trace 2B' E11 Few to no apoptotic cells in control littermates
Specimen 2C E9: embryonic day 9.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS14: prosencephalon Absent 2C E9 No evidence of apoptotic cells.
Specimen 2C' E9: embryonic day 9.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS14: prosencephalon Absent 2C' E9 No evidence of apoptotic cells.
Specimen 2C E10: embryonic day 10.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: telencephalon Present Regionally restricted 2C E10 Numerous apoptotic cells, with highest concentration along ventral telencephalon.
Specimen 2C' E10: embryonic day 10.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: telencephalon Present Regionally restricted 2C' E10 Numerous apoptotic cells, with highest concentration along ventral telencephalon.
Specimen 2C E11: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: telencephalon Present Regionally restricted 2C E11 Apoptotic cells spread across both medial and lateral ganglionic eminences. Small clusters of expressing cells in dorsal cortex.
Specimen 2C' E11: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: telencephalon Present Regionally restricted 2C' E11 Apoptotic cells spread across both medial and lateral ganglionic eminences. Small clusters of expressing cells in dorsal cortex.
Specimen 3B E9 Control: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: future brain Absent 3B E9 Control No expression in brainstem.
Specimen 3B E9 Mcl-1 CKO: embryonic day 9.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS14: future brain Present Regionally restricted 3B E9 Mcl-1 CKO Small clusters of apoptotic cells in ventral brainstem.
Specimen 3B E10 Control: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: future brain Ambiguous Regionally restricted 3B E10 Control No expression in brainstem
Specimen 3B E10 Mcl-1 CKO: embryonic day 10.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: future brain Present Regionally restricted 3B E10 Mcl-1 CKO Apoptosis spread across ventral to dorsal aspect of brainstem.
Specimen 3B E11 Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: brain Ambiguous Regionally restricted 3B E11 Control No expression in brainstem.
Specimen 3B E11 Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: brain Present Regionally restricted 3B E11 Mcl-1 CKO Extensive apoptosis throughout brainstem, except for more lateral regions and a thin region lining the ventricular surface.
Specimen 3C E9 Control: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: future spinal cord Absent 3C E9 Control No expression in upper spinal cord.
Specimen 3C E9 Mcl-1 CKO: embryonic day 9.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS14: future spinal cord Trace Regionally restricted 3C E9 Mcl-1 CKO Few apoptotic cells in ventral portion of upper spinal cord.
Specimen 3C E10 Control: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: future spinal cord Absent 3C E10 Control No expression in upper spinal cord.
Specimen 3C E10 Mcl-1 CKO: embryonic day 10.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: future spinal cord Present Regionally restricted 3C E10 Mcl-1 CKO Apoptosis expanded to cover ventral third of upper spinal cord, excluding ventral lateral horns.
Specimen 3C E11 Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: future spinal cord Ambiguous Regionally restricted 3C E11 Control No expression in upper spinal cord.
Specimen 3C E11 Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Present Regionally restricted 3C E11 Mcl-1 CKO Apoptosis spread from ventral to all but most dorsal region of supper spinal cord.
Specimen 3D E9 Control: embryonic day 9.0 (more )
Structure Level Pattern Image Note
TS14: future spinal cord Absent 3D E9 Control No expression in upper spinal cord.
Specimen 3D E9 Mcl-1 CKO: embryonic day 9.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS14: future spinal cord Ambiguous Regionally restricted 3D E9 Mcl-1 CKO No expression in lower spinal cord
Specimen 3D E10 Control: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: future spinal cord Absent 3D E10 Control No expression inlower spinal cord.
Specimen 3D E10 Mcl-1 CKO: embryonic day 10.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: future spinal cord Trace Regionally restricted 3D E10 Mcl-1 CKO Few apoptotic cells in venral part of lumbar spinal cord.
Specimen 3D E11 Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: future spinal cord Ambiguous Regionally restricted 3D E11 Control No expression in lower spinal cord.
Specimen 3D E11 Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Present Regionally restricted 3D E11 Mcl-1 CKO Apoptosis spread into dorsal part of lumbar spinal cord.
Specimen 4B Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: forebrain Ambiguous Regionally restricted 4B Control No expression in rostral forebrain.
Specimen 4B Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: forebrain Present Regionally restricted 4B Mcl-1 CKO Numerous apoptotic cells in ventral forebrain, with majority of cells located in medial and lateral ganglionic eminences.
Specimen 4B Mcl-1 CKO/BaxNull: embryonic day 11.0; Baxtm1Sjk/Baxtm1Sjk, Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: forebrain Present Scattered 4B Mcl-1 CKO/BaxNull Few scattered apoptotic cells throughout forebrain.
Specimen 4C Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: forebrain Ambiguous Regionally restricted 4C Control No expression in middle forebrain.
Specimen 4C Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: forebrain Present Regionally restricted 4C Mcl-1 CKO Numerous apoptotic cells in ventral forebrain, with majority of cells located in medial and lateral ganglionic eminences.
Specimen 4C Mcl-1 CKO/BaxNull: embryonic day 11.0; Baxtm1Sjk/Baxtm1Sjk, Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: forebrain Present Scattered 4C Mcl-1 CKO/BaxNull Few scattered apoptotic cells throughout forebrain.
Specimen 4D Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: forebrain Ambiguous Regionally restricted 4D Control No expression in caudal forebrain.
Specimen 4D Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: forebrain Present Regionally restricted 4D Mcl-1 CKO Numerous apoptotic cells in ventral forebrain, with majority of cells located in medial and lateral ganglionic eminences.
Specimen 4D Mcl-1 CKO/BaxNull: embryonic day 11.0; Baxtm1Sjk/Baxtm1Sjk, Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: forebrain Present Scattered 4D Mcl-1 CKO/BaxNull Few scattered apoptotic cells throughout forebrain.
Specimen 5D Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: forebrain Trace Regionally restricted 5D Control Few to no apoptotic cells at level of ganglionic eminence.
Specimen 5D HET: embryonic day 11.0; Mcl1tm3Sjk/Mcl1+, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: forebrain Trace Regionally restricted 5D HET Few to no apoptotic cells at level of ganglionic eminence.
Specimen 5D Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: forebrain Present 5D Mcl-1 CKO Significantly more apoptotic cells than all other genotypes.
Specimen 5D Mcl-1 CKO/BaxNull: embryonic day 11.0; Baxtm1Sjk/Baxtm1Sjk, Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: forebrain Trace 5D Mcl-1 CKO/BaxNull No significant difference compared to control and Het.
Specimen 5E Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: forebrain Trace Regionally restricted 5E Control merged , 5E Control Few to no apoptotic cells in lateral ganglionic eminence.
Specimen 5E HET: embryonic day 11.0; Mcl1tm3Sjk/Mcl1+, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: forebrain Trace Regionally restricted 5E HET merged , 5E HET Few to no apoptotic cells in lateral ganglionic eminence.
Specimen 5E Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: forebrain Present 5E Mcl-1 CKO merged , 5E Mcl-1 CKO Significantly more apoptotic cells than all other genotypes.
Specimen 5E Mcl-1 CKO/BaxNull: embryonic day 11.0; Baxtm1Sjk/Baxtm1Sjk, Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: forebrain Trace 5E Mcl-1 CKO/BaxNull , 5E Mcl-1 CKO/BaxNull merged No significant difference compared to control and Het.
Specimen 6B Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: brain Trace Regionally restricted 6B Control Few apoptotic cells in ventral brainstem.
Specimen 6B Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: brain Present Widespread 6B Mcl-1 CKO Widespread apoptosis throughout brainstem.
Specimen 6B Mcl-1 CKO; BaxNull: embryonic day 11.0; Baxtm1Sjk/Baxtm1Sjk, Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: brain Present Clusters 6B Mcl-1 CKO;BaxNull Clusters of apoptotic cells in dorsomedial region of brainstem.
Specimen 6C Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: future spinal cord Trace Regionally restricted 6C Control Few apoptotic cells in cervical spinal cord.
Specimen 6C Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Present Widespread 6C Mcl-1 CKO Apoptosis extended across ventral to dorsal spinal cord at each level
Specimen 6C Mcl-1 CKO; BaxNull: embryonic day 11.0; Baxtm1Sjk/Baxtm1Sjk, Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Ambiguous Regionally restricted 6C Mcl-1 CKO;BaxNull Authors report neural progenitor cells were rescued at cervical level of spinal cord.
Specimen 6D Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: future spinal cord Trace Regionally restricted 6D Control Few apoptotic cells in thoracic spinal cord.
Specimen 6D Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Present Widespread 6D Mcl-1 CKO Apoptosis extended across ventral to dorsal spinal cord at each level.
Specimen 6D Mcl-1 CKO; BaxNull: embryonic day 11.0; Baxtm1Sjk/Baxtm1Sjk, Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Present Clusters 6D Mcl-1 CKO;BaxNull Clusters of apoptotic cells in ventral thoracic spinal cord.
Specimen 6E Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: future spinal cord Trace Regionally restricted 6E Control Few apoptotic cells in lumbar spinal cord,
Specimen 6E Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Present Widespread 6E Mcl-1 CKO Apoptosis extended across ventral to dorsal spinal cord at each level.
Specimen 6E Mcl-1 CKO; BaxNull: embryonic day 11.0; Baxtm1Sjk/Baxtm1Sjk, Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Ambiguous Regionally restricted 6E Mcl-1 CKO/BaxNull Authors report neural progenitor cells were rescued at lumbar level of spinal cord.
Specimen 7D Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: future spinal cord Trace Regionally restricted 7D Control Little to no apoptosis in ventral spinal cord.
Specimen 7D Mcl-1 Het: embryonic day 11.0; Mcl1tm3Sjk/Mcl1+, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Trace Regionally restricted 7D Mcl-1 Het Little to no apoptosis in ventral spinal cord.
Specimen 7D Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Present Regionally restricted 7D Mcl-1 CKO Expression in ventral spinal cord.
Specimen 7D Mcl-1 CKO/BaxNul: embryonic day 11.0; Baxtm1Sjk/Baxtm1Sjk, Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Weak 7D Mcl-1 CKO/BaxNull Authors report 50% rescue of neural progenitor cells.
Specimen 7E Control: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS18: future spinal cord Trace Regionally restricted 7E Control merged , 7E Control cCasp-3 Little to no apoptosis in ventral spinal cord.
Specimen 7E Mcl-1 Het: embryonic day 11.0; Mcl1tm3Sjk/Mcl1+, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Trace Regionally restricted 7E Mcl-1 Het cCasp-3 , 7E Mcl-1 Het merged Little to no apoptosis in ventral spinal cord.
Specimen 7E Mcl-1 CKO: embryonic day 11.0; Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Present Regionally restricted 7E Mcl-1 CKO cCasp-3 , 7E Mcl-1 CKO merged Expression in ventral spinal cord.
Specimen 7E Mcl-1 CKO/BaxNull: embryonic day 11.0; Baxtm1Sjk/Baxtm1Sjk, Mcl1tm3Sjk/Mcl1tm3Sjk, Tg(Nes-cre)2472Pick/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS18: future spinal cord Weak 7E Mcl-1 CKO/BaxNull cCasp-3 , 7E Mcl-1 CKO/BaxNull merged Authors report 50% rescue of neural progenitor cells.
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03/24/2026
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