Gli3 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:168038 Bimonte S, et al., Ofd1 is required in limb bud patterning and endochondral bone development. Dev Biol. 2011 Jan 15;349(2):179-91
Assay type:	Western blot
MGI Accession ID:	MGI:7258493
Gene symbol:	Gli3
Gene name:	GLI-Kruppel family member GLI3

Antibody:

Gli3ab1

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Genetic Background	Mutant Allele(s)	Sex	Note
WT fl	E11.5	TS19: forelimb bud	Present	Present	involves: 129S2/SvPas * C57BL/6J * SJL/J		Not Specified
WT hl	E11.5	TS19: hindlimb bud	Present	Present	involves: 129S2/SvPas * C57BL/6J * SJL/J		Not Specified
Ofd1fl/+Prx1Cre fl	E11.5	TS19: forelimb bud	Present	Present	involves: 129S2/SvPas * C57BL/6J * SJL/J	Ofd1^tm2.1Bfra/Ofd1⁺, Tg(Prrx1-cre)1Cjt/0	Female	Conditional mutant.
Ofd1fl/+Prx1Cre hl	E11.5	TS19: hindlimb bud	Present	Present	involves: 129S2/SvPas * C57BL/6J * SJL/J	Ofd1^tm2.1Bfra/Ofd1⁺, Tg(Prrx1-cre)1Cjt/0	Female	Conditional mutant.
Ofd1fl/yPrx1Cre fl	E11.5	TS19: forelimb bud	Weak (c)	Very strong (d)	involves: 129S2/SvPas * C57BL/6J * SJL/J	Ofd1^tm2.1Bfra/Y, Tg(Prrx1-cre)1Cjt/0	Male	Conditional mutant.
Ofd1fl/yPrx1Cre hl	E11.5	TS19: hindlimb bud	Weak (c)	Strong (e)	involves: 129S2/SvPas * C57BL/6J * SJL/J	Ofd1^tm2.1Bfra/Y, Tg(Prrx1-cre)1Cjt/0	Male	Conditional mutant.

Notes:
(a) Gli3R (truncated repressor form)
(b) Gli3A (full-length form)
(c) Amount of repressor form was reduced compared to wild type.
(d) Increased in activator form most evident in forelimb of male mutants.
(e) Amount of activator form was dramatically increased compared with wildtype.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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