Gene Expression Data
Assay Details
Assay
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Reference:
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J:168038
Bimonte S, et al., Ofd1 is required in limb bud patterning and endochondral bone development. Dev Biol. 2011 Jan 15;349(2):179-91
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7258480
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Gene symbol:
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Pth1r
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Gene name:
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parathyroid hormone 1 receptor
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Probe:
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PPR probe3
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
8D:
embryonic day 16.5
(more )
Specimen
8D:
(close )
Genetic Background:
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involves: 129S2/SvPas * C57BL/6J * SJL/J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: ulna |
growth plate cartilage chondrocyte |
Present |
Regionally restricted |
8D
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In proximal ulna, expression in prehypertrophic chondrocytes.
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TS24: perichondrium |
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Present |
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8D
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Specimen
8E:
(close )
Genetic Background:
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involves: 129S2/SvPas * C57BL/6J * SJL/J
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Mutant Allele(s):
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Ofd1tm2.1Bfra/Ofd1+ Tg(Prrx1-cre)1Cjt/0
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Age:
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embryonic day 16.5
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: ulna |
growth plate cartilage chondrocyte |
Present |
Regionally restricted |
8E
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Normal expression in prehypertrophic chondrocytes compared to wildtype.
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TS24: perichondrium |
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Weak |
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8E
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Reduced expression in perichondrium.
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Specimen
8F:
(close )
Genetic Background:
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involves: 129S2/SvPas * C57BL/6J * SJL/J
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Mutant Allele(s):
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Ofd1tm2.1Bfra/Y Tg(Prrx1-cre)1Cjt/0
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Age:
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embryonic day 16.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: ulna |
growth plate cartilage chondrocyte |
Present |
Regionally restricted |
8F
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Normal expression in prehypertrophic chondrocytes compared to wildtype.
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TS24: perichondrium |
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Trace |
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8F
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Expression almost absent in perichondrium.
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