Probe:	Calm3 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	1E	Expressed in the posterior extramural stream, which crossed the midline and reached the contralateral prospective lateral reticular nucleus region. Expression was relatively low compared with its surroundings.
TS22: hindbrain	Present		1E	Expressed in most hindbrain areas, with relatively strong expression in the brainstem motor nuclei.
TS22: brainstem nucleus	Present		1E
TS22: hindbrain floor plate	Absent		1E

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted	1J	Expressed in the anterior extramural stream, which just reached the ipsilateral prospective pontine nucleus region.
TS22: brainstem nucleus	Present		1J
TS22: hindbrain	Present		1J	Expressed in most hindbrain areas, with relatively strong expression in the brainstem motor nuclei.
TS22: hindbrain floor plate	Absent		1J

Structure	Level	Pattern	Image	Note
TS25: medulla oblongata	Present	Regionally restricted	1O	Expressed in the posterior extramural stream, which gives rise to the external cuneate nucleus.

Structure	Level	Pattern	Image	Note
TS25: medulla oblongata	Present	Regionally restricted	1S	Expressed in the posterior extramural stream, which gives rise to the lateral reticular nucleus.

Structure	Level	Pattern	Image	Note
TS25: pons	Present	Regionally restricted	1W	Expressed in the anterior extramural stream, which gives rise to the pontine nucleus.