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Gene Expression Data
Assay Details
Assay
Reference: J:248980 Guo H, et al., Trinucleotide repeat containing 6c (TNRC6c) is essential for microvascular maturation during distal airspace sacculation in the developing lung. Dev Biol. 2017 Oct 1;430(1):214-223
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7258080
Gene symbol: Nkx2-1
Gene name: NK2 homeobox 1
Antibody: Anti-Thyroid Transcription Factor (TTF-1) Clone 8G7G3/1
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3B E18.5 WT: embryonic day 18.5 (more )
Note: Double labeled: red - Nkx2-1; blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS26: lung alveolus pulmonary alveolar epithelial cell Present Spotted 3B E18.5 WT Nkx2.1 , 3B E18.5 WT +DAPI Salt-and-pepper pattern of expression in epithelial cells nuclei in distal lung.
Specimen 3B E18.5 TNRC6c-/-: embryonic day 18.5; Tnrc6cGt(IST11346C2)Tigm/Tnrc6cGt(IST11346C2)Tigm (more )
Note: Double labeled: red - Nkx2-1; blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS26: lung alveolus pulmonary alveolar epithelial cell Present Spotted 3B E18.5 TNRC6c-/- +DAPI , 3B E18.5 TNRC6c-/- Nkx2.1 Expression pattern similar in wildtype and mutant lungs.
Specimen 3B P0 WT: postnatal day 0 (more )
Note: Double labeled: red - Nkx2-1; blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: lung alveolus pulmonary alveolar epithelial cell Present Spotted 3B PN0 WT Nkx2.1 , 3B PN0 WT +DAPI Salt-and-pepper pattern of expression in epithelial cells nuclei in distal lung.
Specimen 3B P0 TNRC6c-/-: postnatal day 0; Tnrc6cGt(IST11346C2)Tigm/Tnrc6cGt(IST11346C2)Tigm (more )
Note: Double labeled: red - Nkx2-1; blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS27: lung alveolus pulmonary alveolar epithelial cell Present Spotted 3B PN0 TNRC6c-/- Nkx2.1 , 3B PN0 TNRC6c-/- +DAPI Expression pattern similar in wildtype and mutant lungs.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory