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Gene Expression Data
Assay Details
Assay
Reference: J:180785 Loebel DA, et al., Regionalized Twist1 activity in the forelimb bud drives the morphogenesis of the proximal and preaxial skeleton. Dev Biol. 2012 Feb 15;362(2):132-40
Assay type: RNA in situ
MGI Accession ID: MGI:7256601
Gene symbol: Twist1
Gene name: twist basic helix-loop-helix transcription factor 1
Probe: Twist1 probe6
Probe preparation: labelled with digoxigenin RNA
Results
Specimen S2A: embryonic day 9.5; Twist1tm2Bhr/Twist1+ (more )
Structure Level Pattern Image Note
TS15: forelimb bud Present Widespread S2A Expression throughout limb bud.
Specimen S2B: embryonic day 9.5; Mesp1tm2(cre)Ysa/Mesp1+, Twist1tm2Bhr/Twist1tm2.1Bhr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: forelimb bud Present Regionally restricted S2B Expression reduced in anterior and proximal limb bud.
Specimen S2C: embryonic day 10.5; Twist1tm2Bhr/Twist1+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted S2C Strongest expression around periphery of limb bud.
Specimen S2D: embryonic day 10.5; Mesp1tm2(cre)Ysa/Mesp1+, Twist1tm2Bhr/Twist1tm2.1Bhr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: forelimb bud Present Regionally restricted S2D Expression clearly reduced at anterior margin of limb bud.
Specimen S3G: embryonic day 9.5; Twist1tm2Bhr/Twist1+ (more )
Structure Level Pattern Image Note
TS15: embryo mesenchyme Strong Regionally restricted S3G Strong expression in region of cervical mesoderm anterior to forelimb buds.
Specimen S3H: embryonic day 9.5; Mesp1tm2(cre)Ysa/Mesp1+, Twist1tm2Bhr/Twist1tm2.1Bhr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS15: embryo mesenchyme Weak Regionally restricted S3H Reduced expression in region of cervical mesoderm anterior to forelimb buds.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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