Gene Expression Data
Assay Details
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Assay
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Reference:
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J:320853
Mitomo K, et al., Hypoplasia of medial pterygoid process in sphenoid bone relates to decreased mesenchymal cell proliferation in the Runx2-haploinsufficient cleidocranial dysplasia mouse model. Arch Oral Biol. 2022 Mar;135:105358
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6888387
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Gene symbol:
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Pcna
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Gene name:
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proliferating cell nuclear antigen
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Antibody:
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anti-PCNA (16D10)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
2C/E:
embryonic day 14.5
(more  )
Note: Double labeled: green - PCNA; red - Runx2 (assay MGI:6888384).
Specimen
2C/E:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - PCNA; red - Runx2 (assay MGI:6888384).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: chondrocranium cartilage condensation |
Present |
Regionally restricted |
2C
,
2E
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Co-expression with Runx2 in numerous cells in mesenchymal cell condensation of medial pterygoid process of sphenoid bone.
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| TS22: head mesenchyme |
Present |
Regionally restricted |
2C
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2E
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Expression in cells outside of mesenchymal cell condensation region, not expressing Runx2.
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| TS22: sphenoid bone |
Present |
Regionally restricted |
2C
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2E
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Co-expression with Runx2 at end of sphenoid bone.
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Specimen
2D/F:
embryonic day 14.5; Runx2tm1Kish/Runx2 +
(more )
Note: Double labeled: green - PCNA; red - Runx2 (assay MGI:6888384).
Specimen
2D/F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Runx2tm1Kish/Runx2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - PCNA; red - Runx2 (assay MGI:6888384).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: chondrocranium cartilage condensation |
Present |
Regionally restricted |
2F
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2D
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Compared to Runx2+/+ mice, smaller number and distribution of co-expressing cells in mesenchymal cell condensation of medial pterygoid process region of sphenoid bone.
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| TS22: sphenoid bone |
Present |
Regionally restricted |
2F
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2D
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Co-expression with Runx2 at end of sphenoid bone.
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Analysis Tools