Gene Expression Data
Assay Details
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Assay
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Reference:
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J:320853
Mitomo K, et al., Hypoplasia of medial pterygoid process in sphenoid bone relates to decreased mesenchymal cell proliferation in the Runx2-haploinsufficient cleidocranial dysplasia mouse model. Arch Oral Biol. 2022 Mar;135:105358
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6888385
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Gene symbol:
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Pcna
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Gene name:
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proliferating cell nuclear antigen
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Antibody:
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anti-PCNA (M0879)
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
1G:
embryonic day 14.5
(more  )
Specimen
1G:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: chondrocranium cartilage condensation |
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Present |
Regionally restricted |
1G
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Expression in several cells scattered throughout mesenchymal cell condensation of medial pterygoid process region of sphenoid bone.
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| TS22: head mesenchyme |
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Present |
Regionally restricted |
1G
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Expression in mesenchymal cells surrounding the condensation region.
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| TS22: sphenoid bone |
chondrocyte |
Present |
Regionally restricted |
1G
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Expression in many chondrocytes at lateral end of sphenoid bone.
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Specimen
1H:
embryonic day 14.5; Runx2tm1Kish/Runx2 +
(more )
Specimen
1H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Runx2tm1Kish/Runx2+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: head mesenchyme |
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Present |
Regionally restricted |
1H
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Expression in mesenchymal cells beneath lateral end of sphenoid bond.
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| TS22: sphenoid bone |
chondrocyte |
Present |
Regionally restricted |
1H
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Expression in many chondrocytes at lateral end of sphenoid bone.
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Specimen
4A:
embryonic day 18.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: chondrocranium cartilage condensation |
Present |
Regionally restricted |
4A
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Expression in substantial number of cells in mesenchymal condensation of future medial pterygoid process.
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Specimen
4B:
embryonic day 18.5; Runx2tm1Kish/Runx2 +
(more )
Specimen
4B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Runx2tm1Kish/Runx2+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: chondrocranium cartilage condensation |
Present |
Regionally restricted |
4B
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Expression in fewer cells in future medial pterygoid process area than in Runx2+/+, but difference was not significant
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Specimen
4C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Runx2tm1Kish/Runx2tm1Kish
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: chondrocranium cartilage condensation |
Weak |
Regionally restricted |
4C
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Expression in few cells more sparcely distributed in mesenchymal cell condensation of future medial pterygoid process.
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Analysis Tools
