Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:320853
Mitomo K, et al., Hypoplasia of medial pterygoid process in sphenoid bone relates to decreased mesenchymal cell proliferation in the Runx2-haploinsufficient cleidocranial dysplasia mouse model. Arch Oral Biol. 2022 Mar;135:105358
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6888384
|
|
Gene symbol:
|
Runx2
|
|
Gene name:
|
runt related transcription factor 2
|
|
Antibody:
|
anti-Runx2 (12556)
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 555
|
|
|
Results
|
Specimen
2A/E:
embryonic day 14.5
(more  )
Note: Double labeled: red - Runx2; green - Pcna (assay MGI:6888387).
Specimen
2A/E:
(close  )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Runx2; green - Pcna (assay MGI:6888387).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: chondrocranium cartilage condensation |
Present |
Regionally restricted |
2E
,
2A
|
Co-expression with Pcna in numerous cells in mesenchymal cell condensation of medial pterygoid process of sphenoid bone.
|
| TS22: head mesenchyme |
Present |
Regionally restricted |
2E
,
2A
|
Expression in many mesenchymal cells located between cartilage of lateral end of sphenoid bone and mesenchymal condensation for medial pterygoid process, not expressing Pcna.
|
| TS22: sphenoid bone |
Present |
Regionally restricted |
2A
,
2E
|
Co-expression with Pcna at end of sphenoid bone.
|
Specimen
2B/F:
embryonic day 14.5; Runx2tm1Kish/Runx2 +
(more )
Note: Double labeled: red - Runx2; green - Pcna (assay MGI:6888387).
Specimen
2B/F:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd
|
|
Mutant Allele(s):
|
Runx2tm1Kish/Runx2+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Runx2; green - Pcna (assay MGI:6888387).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: head mesenchyme |
Present |
Regionally restricted |
2B
,
2F
|
Compared to Runx2+/+ mice, smaller number and distribution of co-expressing cells in mesenchymal cell condensation of medial pterygoid process region of sphenoid bone.
|
| TS22: sphenoid bone |
Present |
Regionally restricted |
2F
,
2B
|
Co-expression with Pcna at end of sphenoid bone.
|
|
Analysis Tools