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Gene Expression Data
Assay Details
Assay
Reference: J:301700 Cao H, et al., Temporal and tissue specific regulation of RP-associated splicing factor genes PRPF3, PRPF31 and PRPC8--implications in the pathogenesis of RP. PLoS One. 2011 Jan 19;6(1):e15860
Assay type: RT-PCR
MGI Accession ID: MGI:6888207
Gene symbol: Prpf8
Gene name: pre-mRNA processing factor 8
Probe: Prpc8-pA, Prpc8-pB
Assay notes: Quantitative RT-PCR. Relative expression was normalized to 18S. Dark blue diamond, liver; pink, lung; yellow, kidney; light blue x, heart; purple, spleen; red, brain; green line, retina; dark blue horizontal line, muscle; light blue horizontal line, intestine.
Results
Image: 3b ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
E15.5 liver E15.5 TS23: liver Present 1 µg; total RNA CD-1 Not Specified
E15.5 lung E15.5 TS23: lung Present 1 µg; total RNA CD-1 Not Specified
E15.5 kidney E15.5 TS23: metanephros Present 1 µg; total RNA CD-1 Not Specified
E15.5 heart E15.5 TS23: heart Present 1 µg; total RNA CD-1 Not Specified
E15.5 spleen E15.5 TS23: spleen primordium Present 1 µg; total RNA CD-1 Not Specified
E15.5 brain E15.5 TS23: brain Present 1 µg; total RNA CD-1 Not Specified
E15.5 retina E15.5 TS23: retina Present 1 µg; total RNA CD-1 Not Specified
E15.5 muscle E15.5 TS23: muscle tissue Present 1 µg; total RNA CD-1 Not Specified
E15.5 intestine E15.5 TS23: intestine Present 1 µg; total RNA CD-1 Not Specified
5d liver P5 TS28: liver Present 1 µg; total RNA CD-1 Not Specified
5d lung P5 TS28: lung Present 1 µg; total RNA CD-1 Not Specified
5d kidney P5 TS28: metanephros Present 1 µg; total RNA CD-1 Not Specified
5d heart P5 TS28: heart Present 1 µg; total RNA CD-1 Not Specified
5d spleen P5 TS28: spleen Present 1 µg; total RNA CD-1 Not Specified
5d brain P5 TS28: brain Present 1 µg; total RNA CD-1 Not Specified
5d retina P5 TS28: retina Present 1 µg; total RNA CD-1 Not Specified
5d muscle P5 TS28: muscle tissue Present 1 µg; total RNA CD-1 Not Specified
5d intestine P5 TS28: intestine Present 1 µg; total RNA CD-1 Not Specified
1m liver P m 1 TS28: liver Present 1 µg; total RNA CD-1 Not Specified
1m lung P m 1 TS28: lung Present 1 µg; total RNA CD-1 Not Specified
1m kidney P m 1 TS28: metanephros Present 1 µg; total RNA CD-1 Not Specified
1m heart P m 1 TS28: heart Present 1 µg; total RNA CD-1 Not Specified
1m spleen P m 1 TS28: spleen Present 1 µg; total RNA CD-1 Not Specified
1m brain P m 1 TS28: brain Present (a) 1 µg; total RNA CD-1 Not Specified
1m retina P m 1 TS28: retina Strong 1 µg; total RNA CD-1 Not Specified
1m muscle P m 1 TS28: muscle tissue Present 1 µg; total RNA CD-1 Not Specified
1m intestine P m 1 TS28: intestine Present 1 µg; total RNA CD-1 Not Specified
4m liver P m 4 TS28: liver Present 1 µg; total RNA CD-1 Not Specified
4m lung P m 4 TS28: lung Present 1 µg; total RNA CD-1 Not Specified
4m kidney P m 4 TS28: metanephros Present 1 µg; total RNA CD-1 Not Specified
4m heart P m 4 TS28: heart Present 1 µg; total RNA CD-1 Not Specified
4m spleen P m 4 TS28: spleen Present 1 µg; total RNA CD-1 Not Specified
4m brain P m 4 TS28: brain Present (a) 1 µg; total RNA CD-1 Not Specified
4m retina P m 4 TS28: retina Strong 1 µg; total RNA CD-1 Not Specified
4m muscle P m 4 TS28: muscle tissue Present 1 µg; total RNA CD-1 Not Specified
4m intestine P m 4 TS28: intestine Present 1 µg; total RNA CD-1 Not Specified
1y liver P y 1 TS28: liver Present 1 µg; total RNA CD-1 Not Specified
1y lung P y 1 TS28: lung Present 1 µg; total RNA CD-1 Not Specified
1y kidney P y 1 TS28: metanephros Present 1 µg; total RNA CD-1 Not Specified
1y heart P y 1 TS28: heart Present 1 µg; total RNA CD-1 Not Specified
1y spleen P y 1 TS28: spleen Present 1 µg; total RNA CD-1 Not Specified
1y brain P y 1 TS28: brain Present 1 µg; total RNA CD-1 Not Specified
1y retina P y 1 TS28: retina Present 1 µg; total RNA CD-1 Not Specified
1y muscle P y 1 TS28: muscle tissue Present 1 µg; total RNA CD-1 Not Specified
1y intestine P y 1 TS28: intestine Present 1 µg; total RNA CD-1 Not Specified
Notes:
(a) The levels of mRNA in the brain are 34-36% of that detected in the retina.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/09/2024
MGI 6.23 		The Jackson Laboratory