About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:316590 Rybtsov S, et al., Tracing the origin of the HSC hierarchy reveals an SCF-dependent, IL-3-independent CD43(-) embryonic precursor. Stem Cell Reports. 2014 Sep 9;3(3):489-501
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6887672
Gene symbol: Ptprc
Gene name: protein tyrosine phosphatase receptor type C
Antibody: anti-CD45
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 3C: embryonic day 10.0 (more )
Note: Triple labeled: blue - CD45 (Ptprc); green - VE-cadherin (Cdh5 assay MGI:6887669); red - CD43 (Spn assay MGI:6887671).
Structure Level Pattern Image Note
TS16: dorsal aorta Ambiguous Regionally restricted 3C Merge , 3C CD45 No expression in small ventrally-located intra-aortic clusters.
Specimen 3D: embryonic day 10.0 (more )
Note: Triple labeled: blue - CD45 (Ptprc); green - VE-cadherin (Cdh5 assay MGI:6887669); red - CD43 (Spn assay MGI:6887671).
Structure Level Pattern Image Note
TS16: dorsal aorta Present Regionally restricted 3D CD45 , 3D Merge Expression in rare cells in small intra-aortic clusters. No expression in large clusters in subendothelial (aortic) mesenchyme.
Specimen S3A: embryonic day 10.5 (more )
Note: Triple labeled: blue - CD45 (Ptprc); green - VE-cadherin (Cdh5 assay MGI:6887669); red - CD43 (Spn assay MGI:6887671).
Structure Level Pattern Image Note
TS17: umbilical artery Ambiguous Regionally restricted S3A CD45 , S3A Merge No expression in some hematopoietic clusters.
Specimen S3B: embryonic day 10.5 (more )
Note: Triple labeled: blue - CD45 (Ptprc); green - VE-cadherin (Cdh5 assay MGI:6887669); red - CD43 (Spn assay MGI:6887671).
Structure Level Pattern Image Note
TS17: umbilical artery Present Regionally restricted S3B CD45 , S3B Merge Expression in some apically-located hematopoietic clusters.
Specimen S3C: embryonic day 10.5 (more )
Note: Triple labeled: blue - CD45 (Ptprc); green - VE-cadherin (Cdh5 assay MGI:6887669); red - CD43 (Spn assay MGI:6887671).
Structure Level Pattern Image Note
TS17: vitelline artery Present Regionally restricted S3C Merge , S3C CD45 Expression in large hematopoietic clusters adhered to luminal surface.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory