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Gene Expression Data
Assay Details
Assay
Reference: J:316590 Rybtsov S, et al., Tracing the origin of the HSC hierarchy reveals an SCF-dependent, IL-3-independent CD43(-) embryonic precursor. Stem Cell Reports. 2014 Sep 9;3(3):489-501
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6887671
Gene symbol: Spn
Gene name: sialophorin
Antibody: anti-CD43 (M19)
Detection system: Secondary antibody coupled to other - see notes
Assay notes: Anti-goat secondary antibody labelled with NL557.
Results
Specimen 3A: embryonic day 9.0 (more )
Note: Triple labeled: red - CD43 (Spn); green - VE-cadherin (Cdh5 assay MGI:6887669); blue - Runx1 (assay MGI:6887670).
Structure Level Pattern Image Note
TS14: dorsal aorta Present Regionally restricted 3A Merge , 3A CD43 Expression in cells localized to ventral floor of dorsal aorta.
Specimen 3A': embryonic day 9.0 (more )
Note: Triple labeled: red - CD43 (Spn); green - VE-cadherin (Cdh5 assay MGI:6887669); blue - Runx1 (assay MGI:6887670).
Structure Level Pattern Image Note
TS14: dorsal aorta Ambiguous Regionally restricted 3A' CD43 , 3A' Merge No expression in pro-hematopoietic stem cells (pro-HSCs) localized to ventral endothelium.
Specimen 3B: embryonic day 9.0 (more )
Note: Triple labeled: red - CD43 (Spn); green - VE-cadherin (Cdh5 assay MGI:6887669); blue - Runx1 (assay MGI:6887670).
Structure Level Pattern Image Note
TS14: vitelline artery Present Regionally restricted 3B Merge , 3B CD43 Expression in strings of large clusters.
TS14: blood vessel Present Regionally restricted 3B CD43 , 3B Merge No expression in small vessels connecting omphalomesenteric artery and dorsal aorta.
Specimen 3B': embryonic day 9.0 (more )
Note: Triple labeled: red - CD43 (Spn); green - VE-cadherin (Cdh5 assay MGI:6887669); blue - Runx1 (assay MGI:6887670).
Structure Level Pattern Image Note
TS14: vitelline artery Present Regionally restricted 3B' CD43 , 3B' Merge Expression in large clusters inside omphalomesenteric (vitelline) artery.
Specimen 3C: embryonic day 10.0 (more )
Note: Triple labeled: red - CD43 (Spn); green - VE-cadherin (Cdh5 assay MGI:6887669); blue - CD45 (Ptprc assay MGI:6887672).
Structure Level Pattern Image Note
TS16: dorsal aorta Present Regionally restricted 3C Merge , 3C CD43 Expression in small ventrally-located intra-aortic clusters.
Specimen 3D: embryonic day 10.0 (more )
Note: Triple labeled: red - CD43 (Spn); green - VE-cadherin (Cdh5 assay MGI:6887669); blue - CD45 (Ptprc assay MGI:6887672).
Structure Level Pattern Image Note
TS16: dorsal aorta Present Regionally restricted 3D Merge , 3D CD43 Expression in small intra-aortic clusters. Expression in large clusters in subendothelial (aortic) mesenchyme.
Specimen 3E: embryonic day 10.0 (more )
Note: Triple labeled: red - CD43 (Spn); green - VE-cadherin (Cdh5 assay MGI:6887669); blue - Runx1 (assay MGI:6887670).
Structure Level Pattern Image Note
TS16: dorsal aorta Present Regionally restricted 3E CD43 , 3E Merge Expression in intra-aortic clusters.
Specimen S3A: embryonic day 10.5 (more )
Note: Triple labeled: red - CD43 (Spn); green - VE-cadherin (Cdh5 assay MGI:6887669); blue - CD45 (Ptprc assay MGI:6887672).
Structure Level Pattern Image Note
TS17: umbilical artery Present Regionally restricted S3A CD43 , S3A Merge Expression in hematopoietic clusters.
Specimen S3B: embryonic day 10.5 (more )
Note: Triple labeled: red - CD43 (Spn); green - VE-cadherin (Cdh5 assay MGI:6887669); blue - CD45 (Ptprc assay MGI:6887672).
Structure Level Pattern Image Note
TS17: umbilical artery Present Regionally restricted S3B CD43 , S3B Merge Expression in many apically-located hematopoietic clusters.
Specimen S3C: embryonic day 10.5 (more )
Note: Triple labeled: red - CD43 (Spn); green - VE-cadherin (Cdh5 assay MGI:6887669); blue - CD45 (Ptprc assay MGI:6887672).
Structure Level Pattern Image Note
TS17: vitelline artery Present Regionally restricted S3C CD43 , S3C Merge Expression in large hematopoeitic clusters adhered to luminal surface.
Specimen S3D: embryonic day 9.5 (more )
Note: Triple labeled: red - CD43 (Spn); green - VE-cadherin (Cdh5 assay MGI:6887669); blue - Runx1 (assay MGI:6887670).
Structure Level Pattern Image Note
TS15: hematopoietic system Present Regionally restricted S3D Merge , S3D CD43 Expression in hematopoietic cells in circulation within dorsal aorta.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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