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Gene Expression Data
Assay Details
Assay
Reference: J:137801 Nakayama A, et al., Expression of the basal cell markers of taste buds in the anterior tongue and soft palate of the mouse embryo. J Comp Neurol. 2008 Jul 10;509(2):211-24
Assay type: RNA in situ
MGI Accession ID: MGI:6885885
Gene symbol: Ascl1
Gene name: achaete-scute family bHLH transcription factor 1
Probe: Mash1 probe20
Probe preparation: Antisense RNA
Visualized with: Fluorescence
Results
Specimen 3A/C: embryonic day 13.5 (more )
Note: Double labeled: pink - Ascl1; green - Shh (assay MGI:6885880).
Structure Level Pattern Image Note
TS21: tongue papillary placode Absent 3C , 3A Expression not observed at E13.5.
Specimen 3E/G: embryonic day 14.5 (more )
Note: Double labeled: pink - Ascl1; green - Shh (assay MGI:6885880).
Structure Level Pattern Image Note
TS22: tongue papillary placode Present Regionally restricted 3G , 3E Expression in a fraction of Shh expressing cells within cell clusters.
Specimen 3I/K: postnatal adult (more )
Note: Double labeled: pink - Ascl1; green - Shh (assay MGI:6885880).
Structure Level Pattern Image Note
TS28: soft palate Present Regionally restricted 3I , 3K Expression in taste buds on soft palate.
Specimen 7A/C: embryonic day 14.5 (more )
Note: Double labeled: pink - Ascl1; green - Shh (assay MGI:6885880).
Structure Level Pattern Image Note
TS22: soft palate Absent 7A , 7C Expression not observed at E14.5.
Specimen E15.5 not shown: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: soft palate Ambiguous Regionally restricted Expression not detected by double color in situ hybridization. In single color analysis, only one expressing cells was observed in >100 Shh-expressing cells prepared from 3 mouse embryos.
Specimen 7E/G: embryonic day 16.5 (more )
Note: Double labeled: pink - Ascl1; green - Shh (assay MGI:6885880).
Structure Level Pattern Image Note
TS24: soft palate Trace Scattered 7E , 7G Expression in a few cells in almost every Shh-expressing cell cluster.
Specimen 7I/K: postnatal adult (more )
Note: Double labeled: pink - Ascl1; green - Shh (assay MGI:6885880).
Structure Level Pattern Image Note
TS28: soft palate Present Regionally restricted 7K , 7I Expression in both basal cells and in elongated cells of taste buds.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory