Antibody:	anti-Ankrd11
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS21: head mesenchyme	Present	Regionally restricted	4A E13.5	Expression is in orofacial regions.
TS21: maxilla	Present	Regionally restricted	4A E13.5	Expression is in a mesenchymal condensation in the developing maxillary region (sphenoid wing).
TS21: forebrain	Present		4A E13.5
TS21: oral epithelium	Present		4A E13.5
TS21: palatal shelf	Present	Regionally restricted	4A E13.5

Structure	Level	Pattern	Image	Note
TS22: mandible	Present	Regionally restricted	4A E14.5	Expression is in ossification centers and at sites of bone development.
TS22: maxilla	Present	Regionally restricted	4A E14.5	Expression is in ossification centers and at sites of bone development.
TS22: molar dental papilla	Present	Regionally restricted	4A E14.5	Expression is in pre-odontoblasts.
TS22: eye anterior segment	Present		4A E14.5
TS22: lens	Present		4A E14.5
TS22: nasal septum	Present		4A E14.5
TS22: cranium bone primordium	Present	Regionally restricted	4A E14.5	Expression is in developing turbinates.
TS22: secondary palate	Present	Regionally restricted	4A E14.5	Expression is the midline epithelial seam of the fusing palate.
TS22: tongue	Present	Regionally restricted	4A E14.5

Structure	Level	Pattern	Image	Note
TS27: oral epithelium	Strong		4A P0
TS27: eye posterior segment	Present		4A P0
TS27: mandible	Trace		4A P0
TS27: maxilla	Trace		4A P0
TS27: tooth	Trace		4A P0

Structure	Level	Pattern	Image	Note
TS22: mandible	Present	Regionally restricted	4B E14.5 mandible	Expression is lining the forming mandibular bone.

Structure	Level	Pattern	Image	Note
TS22: vault of skull	Present	Regionally restricted	4B E14.5 mesenchym. cond.	Expression is in the forming ossification centers.

Structure	Level	Pattern	Image	Note
TS27: mandible	Trace		4B P0 mandible

Structure	Level	Pattern	Image	Note
TS27: chondrocranium	Present	Regionally restricted	4B P0 calvaria	Expression is in cells lining the calvarial bone and is absent in osteocytes within the mature bone.

Structure	Level	Pattern	Image	Note
TS20: palatal shelf epithelium	Present	Regionally restricted	4C E12.5
TS20: palatal shelf mesenchyme	Present	Single cells	4C E12.5	Expression is in a few cells in the buccal aspect toward its tip, as well as in its upper, dorsal aspects.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf epithelium	Present		4C E13.5
TS21: palatal shelf mesenchyme	Present	Regionally restricted	4C E13.5	Expression is in a few cells in the buccal aspect toward its tip, as well as in its upper, dorsal aspects.

Structure	Level	Pattern	Image	Note
TS22: maxilla	Strong		4C E14.5
TS22: nasal cavity epithelium	Strong		4C E14.5
TS22: oral epithelium	Strong		4C E14.5
TS22: palatal shelf epithelium	Present	Regionally restricted	4C E14.5	Expression is strongest in the midline epithelial seam.
TS22: palatal shelf mesenchyme	Present	Regionally restricted	4C E14.5	Expression is in a few cells in the buccal aspect toward its tip, as well as in its upper, dorsal aspects.

Structure	Level	Pattern	Image	Note
TS27: nasal cavity epithelium	Present		4C P0
TS27: oral epithelium	Present		4C P0
TS27: maxilla	Weak	Regionally restricted	4C P0	Expression is in presumptive bone-lining cells.
TS27: secondary palate	Weak	Regionally restricted	4C P0	Expression is in presumptive bone-lining cells.

Structure	Level	Pattern	Image	Note
TS22: secondary palate	Present		7B Ankrd11 E14.5

Structure	Level	Pattern	Image	Note
TS27: secondary palate	Present		7B Ankdr11 P0