Antibody:	anti-Irx3 (ab25703)
Detection system:	horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		1A Irx3

Structure	Level	Pattern	Image	Note
TS20: hindlimb mesenchyme	Absent		1B Irx3	Expression below detectable levels in hindlimb bud mesenchyme

Structure	Level	Pattern	Image	Note
TS21: femur cartilage condensation	Present	Regionally restricted	1C Irx3	Expression in center of cartilage anlage.

Structure	Level	Pattern	Image	Note
TS22: femur cartilage condensation	Strong	Regionally restricted	1D Irx3	Strong expression in center of cartilage anlage.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: femur		Present	Regionally restricted	1E Irx3	Expression in femoral growth plate cartilage.
TS23: long bone epiphyseal plate	growth plate cartilage chondrocyte	Strong	Regionally restricted	1E Irx3	Strong expression in prehypertrophic chondrocytes.
TS23: long bone epiphyseal plate hypertrophic zone	hypertrophic chondrocyte	Strong		1E Irx3	Strong expression in hypertrophic chondrocytes.
TS23: long bone epiphyseal plate proliferative zone	growth plate cartilage chondrocyte	Present	Regionally restricted	1E Irx3	Expression in some parts of proliferating and resting zone chondrocytes.
TS23: long bone epiphyseal plate resting zone	growth plate cartilage chondrocyte	Present	Regionally restricted	1E Irx3	Expression in some parts of proliferating and resting zone chondrocytes.