Gene Expression Data
Assay Details
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Assay
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Reference:
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J:165134
Kim B, et al., Potential role for MATER in cytoplasmic lattice formation in murine oocytes. PLoS One. 2010;5(9):e12587
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6872313
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Gene symbol:
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Nlrp5
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Gene name:
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NLR family, pyrin domain containing 5
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Antibody:
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Anti-Mater
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
1A GV:
postnatal week 4-6
(more  )
Note: Germinal vesicle oocytes. Double labeled: green - Nlrp5; red - Padi6 (assay MGI:6872314).
Specimen
1A GV:
(close  )
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Genetic Background:
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CD-1
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Age:
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postnatal week 4-6
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Sex:
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Female
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Germinal vesicle oocytes. Double labeled: green - Nlrp5; red - Padi6 (assay MGI:6872314).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: primary oocyte |
Strong |
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1A GV Mater
,
1A GV Merge
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Expression colocalized with Padi6 strongly in the cortex and, to a lesser degree, in the cytoplasm of germinal vesicle stage oocytes.
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Specimen
1A 2-cell:
embryonic day 1.0
(more )
Note: Double labeled: green - Nlrp5; red - Padi6 (assay MGI:6872314).
Specimen
1A 2-cell:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Nlrp5; red - Padi6 (assay MGI:6872314).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
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1A 2-cell Merge
,
1A 2-cell Mater
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Co-localization with Padi6 was observed at the non-opposed cortex regions of 2-cell embryos with less co-localization being observed throughout the cytoplasm. Nlrp5 (Mater) staining penetrated deeper into the cytoplasm compared with Padi6.
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Specimen
1A 4-cell:
embryonic day 2.0
(more )
Note: Double labeled: green - Nlrp5; red - Padi6 (assay MGI:6872314).
Specimen
1A 4-cell:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Nlrp5; red - Padi6 (assay MGI:6872314).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Present |
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1A 4-cell Merge
,
1A 4-cell Mater
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Co-localization with Padi6 was observed at the non-opposed cortex regions of 4-cell embryos with less co-localization being observed throughout the cytoplasm. Nlrp5 (Mater) staining penetrated deeper into the cytoplasm compared with Padi6.
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Analysis Tools