Gene Expression Data
Assay Details
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Assay
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Reference:
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J:315732
Meglicki M, et al., Appearance and heterochromatin localization of HP1alpha in early mouse embryos depends on cytoplasmic clock and H3S10 phosphorylation. Cell Cycle. 2012 Jun 1;11(11):2189-205
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6872304
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Gene symbol:
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Cbx5
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Gene name:
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chromobox 5
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Results
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Specimen
2a G1/S:
embryonic day 0.5
(more  )
Note: G1/S transition.
Specimen
2a G1/S:
(close  )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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G1/S transition.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Absent |
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2a'
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No expression in chromatin during entire first cell cycle.
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Specimen
2b mid S:
embryonic day 0.5
(more )
Note: middle and late S phase.
Specimen
2b mid S:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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middle and late S phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Absent |
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2b'
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No expression in chromatin during entire first cell cycle.
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Specimen
2c S/G2:
embryonic day 0.5
(more )
Note: S/G2 phase.
Specimen
2c S/G2:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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S/G2 phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Absent |
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2c'
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No expression in chromatin during entire first cell cycle.
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Specimen
2d Met:
embryonic day 0.5
(more )
Note: metaphase.
Specimen
2d Met:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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metaphase.
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Specimen
2e Ana:
embryonic day 0.5
(more )
Note: anaphase of first cleavage division.
Specimen
2e Ana:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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anaphase of first cleavage division.
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Specimen
2-cell G1 not shown:
embryonic day 1.0
(more )
Note: G1 phase.
Specimen
2-cell G1 not shown:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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G1 phase.
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Specimen
3a G1/S:
embryonic day 1.0
(more )
Note: G1/S transition.
Specimen
3a G1/S:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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G1/S transition.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Absent |
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3a''
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3a'
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Expression not detected at G1/S transition.
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Specimen
3b middle S:
embryonic day 1.0
(more )
Note: middle S phase.
Specimen
3b middle S:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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middle S phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Absent |
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3b''
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3b'
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Expression not detected at middle S phase.
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Specimen
3c late S:
embryonic day 1.0
(more )
Note: late S phase.
Specimen
3c late S:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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late S phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Strong |
Regionally restricted |
3c''
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3c'
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Expression not detected in chromatin until late S-phase. Strong, homogeneous expression in nuclei of both blastomeres.
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Specimen
3d S/G2:
embryonic day 1.0
(more )
Note: S/G2 transition.
Specimen
3d S/G2:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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S/G2 transition.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Regionally restricted |
3d'
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3d''
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At S/G2 transition, well-defined foci of expression in areas of highly condensed chromatin.
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Specimen
S1A G1:
embryonic day 1.0
(more )
Note: S/G2 transition. Double labeled: green - Cbx5; red - H3K9Me3.
Specimen
S1A G1:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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S/G2 transition. Double labeled: green - Cbx5; red - H3K9Me3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Regionally restricted |
S1A merge
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S1A HP1alpha
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At S/G2 transition, well-defined foci of expression in areas of highly condensed chromatin.
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Specimen
S1C HP1alpha:
embryonic day 1.0
(more )
Note: S/G2 transition. Double labeled: green - Cbx5; red - Cenpa (assay MGI:6872307).
Specimen
S1C HP1alpha:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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S/G2 transition. Double labeled: green - Cbx5; red - Cenpa (assay MGI:6872307).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
Regionally restricted |
S1C merge
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S1C HP1alpha
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Foci of expression were connected with centromeres.
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Specimen
3e G2:
embryonic day 1.0
(more )
Note: late G2 phase.
Specimen
3e G2:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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late G2 phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Ambiguous |
Regionally restricted |
3e''
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3e'
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Foci of expression disappeared in late G2 of 2nd cell cycle. Expression diffused from heterochromatin areas.
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Specimen
3f M:
embryonic day 1.0
(more )
Note: metaphase and telophase.
Specimen
3f M:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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metaphase and telophase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Absent |
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3f''
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3f'
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No expression on chromosomes during 2nd mitotic division.
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Specimen
S2a G1:
embryonic day 2.0
(more )
Note: G1 phase.
Specimen
S2a G1:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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G1 phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Absent |
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S2a'
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S2a''
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No expression at the beinning of interphase of 3rd cell cycle.
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Specimen
S2b G2:
embryonic day 2.0
(more )
Note: middle/late S phase.
Specimen
S2b G2:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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middle/late S phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Present |
Regionally restricted |
S2b'
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S2b''
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Expression not detected until S-phase of cell cycle. Foci of expression in areas of highly condensed chromatin.
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Specimen
S2c G2:
embryonic day 2.0
(more )
Note: late G2 phase.
Specimen
S2c G2:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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late G2 phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Present |
Diffuse |
S2c''
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S2c'
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No expression in blastomere nuclei. Expression diffused from heterochromatin areas.
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Specimen
S3a G1:
embryonic day 2.5
(more )
Note: G1 phase.
Specimen
S3a G1:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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G1 phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Absent |
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S3a''
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S3a'
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No expression at the beginning of interphase of 4th cell cycle.
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Specimen
S3b S:
embryonic day 2.5
(more )
Note: middle/late S phase.
Specimen
S3b S:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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middle/late S phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Present |
Regionally restricted |
S3b''
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S3b'
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Expression not detected until S-phase of cell cycle. Foci of expression in areas of highly condensed chromatin.
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Specimen
S3c G2:
embryonic day 2.5
(more )
Note: late G2 phase.
Specimen
S3c G2:
(close )
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Genetic Background:
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involves: CBA * C57BL/6
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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late G2 phase.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 8-cell stage embryo |
Ambiguous |
Diffuse |
S3c''
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S3c'
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No expression in blostomere nuclei. Expression diffused from heterochromatin areas.
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