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Gene Expression Data
Assay Details
Assay
Reference: J:315732 Meglicki M, et al., Appearance and heterochromatin localization of HP1alpha in early mouse embryos depends on cytoplasmic clock and H3S10 phosphorylation. Cell Cycle. 2012 Jun 1;11(11):2189-205
Assay type: RT-PCR
MGI Accession ID: MGI:6872299
Gene symbol: Cbx5
Gene name: chromobox 5
Probe: HP1alpha-pA, HP1alpha-pB
Probe preparation: labelled with SYBR green
Results Image: 1A
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
MII oocyte P adult TS28: secondary oocyte Present Not Specified involves: CBA * C57BL/6 Female
1-cell G1/S E0.5 (a) TS1: 1-cell stage embryo Present (b) Not Specified involves: CBA * C57BL/6 Not Specified
1-cell S/G2 E0.5 (a) TS1: 1-cell stage embryo Present (c) Not Specified involves: CBA * C57BL/6 Not Specified
2-cell G1/S E1.0 (a) TS2: 2-cell stage embryo Present (d) Not Specified involves: CBA * C57BL/6 Not Specified
2-cell late S E1.0 (a) TS2: 2-cell stage embryo Weak (e) Not Specified involves: CBA * C57BL/6 Not Specified
2-cell late G2 E1.0 (a) TS2: 2-cell stage embryo Strong (f) Not Specified involves: CBA * C57BL/6 Not Specified
4-cell G1/S E2.0 (a) TS3: 4-cell stage embryo Present (g) Not Specified involves: CBA * C57BL/6 Not Specified
4-cell S1/G2 E2.0 (a) TS3: 4-cell stage embryo Strong (h) Not Specified involves: CBA * C57BL/6 Not Specified
8-cell S/G2 E2.5 (a) TS3: 8-cell stage embryo Very strong (i) Not Specified involves: CBA * C57BL/6 Not Specified
blastocyst E3.5 (a) TS4: blastocyst Trace (j) Not Specified involves: CBA * C57BL/6 Not Specified
Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Expression present at similar level in zygotes at G1/S phase.
(c) Expression present at similar level in zygotes at late S/G2 phase
(d) Slightly higher level of expression in 2-cell embryos at G2/S phase.
(e) At late S phase, expression level decreased dramatically.
(f) In 2-cell embryos at late G2, expression level increased ~8-fold compared to late S phase.
(g) In 4-cell embryos, expression level lower at G1/S.
(h) In 4-cell embryos, expression level higher in late G2 embryos.
(i) In late 8-cell embryos, expression levels even higher than in 2-cell and 4-cell embryos at G2.
(j) Level of expression was low at blastocyst stage.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory