Probe:	Stra6 cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Regionally restricted	1A	Expression was detected in the proximal and dorsal side of the forelimb bud. Transcript levels were stronger in the proximal regions and gradually decreased toward the distal margin.
TS15: trunk mesenchyme	Present	Regionally restricted	1A	Expression was detected in the flank mesenchyme prior to the appearance of the hindlimb bud.

Structure	Level	Pattern	Image	Note
TS16: trunk mesenchyme	Present	Regionally restricted	1B	Expression was continuous in the flank mesenchyme between the fore- and hindlimb buds.

Structure	Level	Pattern	Image	Note
TS16: hindlimb bud mesenchyme	Present	Regionally restricted	1C Stra6	Expression was restricted to the posterior and proximal mesoderm in the hindlimbs.
TS16: forelimb bud mesenchyme	Present	Regionally restricted	1C Stra6	Expression was detected along the dorsal mesoderm of the forelimb buds except in the most distal region or progress zone.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted	1D Stra6	Expression was restricted to the superficial limb mesenchyme, both dorsally and ventrally, and excluded from the central chondrogenic (precartilaginous) condensations. Expression was not detected in the distal tip. Expression was outside of the progress zone.

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit mesenchyme	Present	Regionally restricted	3A	Expression was detected in the undifferentiated mesenchyme at the tip of the digits.
TS21: hindlimb musculature	Weak		3A
TS21: perichondrium	Strong		3A	Proximal regions showed strong expression in the limb perichondrium.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit mesenchyme	Present	Regionally restricted	3B	Expression was detected in the undifferentiated mesenchyme at the tip of the digits.
TS21: perichondrium	Strong		3B	Proximal regions showed strong expression in the limb perichondrium. Rib perichondrium also showed expression.
TS21: forelimb musculature	Weak		3B

Structure	Level	Pattern	Image	Note
TS24: perichondrium	Present	Regionally restricted	3C	Expression was restricted to very limited concentric zones of the hindlimb perichondrium.
TS24: hindlimb muscle	Weak		3C

Structure	Level	Pattern	Image	Note
TS24: perichondrium	Present	Regionally restricted	3D	Expression was restricted to very limited concentric zones of the femur perichondrium. The labeled areas were located slightly distally to the front of hypertrophic chondrocytes and overlying the cartilage 'elongation zone'.