Gene Expression Data
Assay Details
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Assay
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Reference:
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J:316191
Kasaai B, et al., Regulation of the bone-restricted IFITM-like (Bril) gene transcription by Sp and Gli family members and CpG methylation. J Biol Chem. 2013 May 10;288(19):13278-94
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6865689
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Gene symbol:
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Ifitm5
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Gene name:
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interferon induced transmembrane protein 5
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Antibody:
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anti-Bril
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
10B WT:
embryonic day 17.5
(more  )
Specimen
10B WT:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: tibia |
osteoblast |
Present |
Regionally restricted |
10B WT
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Expression in osteoblasts localized at forming bony collar and in primary ossification centers.
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| TS25: perichondrium |
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Present |
Regionally restricted |
10B WT
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Expression in perichondrium adjacent to layers of prehypertrophic and hypertrophic chondrocytes.
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Specimen
10B KO:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: perichondrium |
Present |
Regionally restricted |
10B KO
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Considerable reduction in domain of expressing cells compared with wildtype, but intensity of expression did not appear qualitatively reduced.
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| TS25: tibia |
Present |
Regionally restricted |
10B KO
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Considerable reduction in domain of expressing cells compared with wildtype, but intensity of expression did not appear qualitatively reduced.
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Specimen
10C WT:
embryonic day 17.5
(more )
Specimen
10C WT:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
10C KO:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: chondrocranium |
Present |
Regionally restricted |
10C KO
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No significant difference between wildtype and knockout.
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