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Gene Expression Data
Assay Details
Assay
Reference: J:237701 Saare M, et al., Expression Pattern and Localization Dynamics of Guanine Nucleotide Exchange Factor RIC8 during Mouse Oogenesis. PLoS One. 2015;10(6):e0129131
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6864139
Gene symbol: Numa1
Gene name: nuclear mitotic apparatus protein 1
Antibody: anti-NuMA
Detection system: Alexa Fluor
Results
Specimen 3B/C: postnatal adult (more )
Note: germinal vesicle stage. Triple labeled: red - NUMA (Numa1); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS28: primary oocyte Present Regionally restricted 3B , 3C Broadly expressed in germinal vesicle.
Specimen 3b/c: postnatal adult (more )
Note: Triple labeled: red - NUMA (Numa1); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS28: primary oocyte Present Regionally restricted 3c , 3b Expression partially co-localizing with RIC8 (Ric8a).
Specimen 3E/F: postnatal adult (more )
Note: germinal vesicle breakdown. Triple labeled: red - NUMA (Numa1); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS28: secondary oocyte Present Regionally restricted 3E , 3F After germinal vesicle breakdown, expression sarting to condense around chromoatin.
Specimen 3e/f: postnatal adult (more )
Note: Triple labeled: red - NUMA (Numa1); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS28: secondary oocyte Present Regionally restricted 3e , 3f Expression much wider compared to that of RIC8 (Ric8a).
Specimen 5B/C: postnatal adult (more )
Note: anaphase/telophase of meiosis I. Triple labeled: red - NUMA (Numa1); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 5B , 5C When chromosomes segregate, expression translocated to spindle midzone.
Specimen 6B/C: embryonic day 0.5 (more )
Note: pronuclear stage. Triple labeled: red - NUMA (Numa1); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 6C , 6B Expression broadly localized in pronucleus.
Specimen 6b/c: embryonic day 0.5 (more )
Note: Triple labeled: red - NUMA (Numa1); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 6b , 6c No overlap with RIC8 (Ric8a).
Specimen 7K/L: embryonic day 1.0 (more )
Note: Triple labeled: red - NUMA (Numa1); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted 7L , 7K Expression in nucleus of blastomeres. No overlap with RIC8 (Ric8a) in cortex region.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory