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Gene Expression Data
Assay Details
Assay
Reference: J:237701 Saare M, et al., Expression Pattern and Localization Dynamics of Guanine Nucleotide Exchange Factor RIC8 during Mouse Oogenesis. PLoS One. 2015;10(6):e0129131
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6864134
Gene symbol: Ric8a
Gene name: RIC8 guanine nucleotide exchange factor A
Antibody: anti-Ric8
Detection system: Alexa Fluor
Results
Specimen 1A: postnatal adult (more )
Note: primordial follicle stage. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte of primordial follicle Present Regionally restricted 1A Expression localized to cytoplasm of primary oocyte.
Specimen 1B: postnatal adult (more )
Note: primary follicle stage. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte of primary follicle Present Regionally restricted 1B Expression localized to cytoplasm of primary oocyte.
Specimen 1C: postnatal adult (more )
Note: preantral secondary follicle. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte of secondary follicle Present Regionally restricted 1C At preantral secondary follicle stage, cytoplasm localization of expression in oocyte.
Specimen 1D: postnatal adult (more )
Note: antral secondary follicle. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte of secondary follicle Present Regionally restricted 1D At antral secondary follicle stage, cytoplasm localization of expression in oocyte. Expression also targeted to germical vesicle.
Specimen 1E: postnatal adult (more )
Note: Graafian follicle. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte of mature follicle Present Regionally restricted 1E At preovulatory stage, expression localized in germinal vesicle. Expression accumulates from cytoplasm to cell cortex.
TS28: cumulus of mature follicle Present Regionally restricted 1E Expression also present at detectable level in cytoplasm of surrounding cumulus cells
Specimen 1F: postnatal adult (more )
Note: Graafian follicle. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte of mature follicle Present Regionally restricted 1F Expression accumulates from cytoplasm to cell cortex.
TS28: cumulus of mature follicle Present Regionally restricted 1F Expression also present at detectable level in cytoplasm of surrounding cumulus cells and mural granulosa cells.
TS28: mural of mature follicle Present Regionally restricted 1F Expression also present at detectable level in cytoplasm of surrounding cumulus cells and mural granulosa cells.
Specimen 1G: postnatal adult (more )
Note: Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Structure Level Pattern Image Note
TS28: oviduct Present Widespread 1G Expression in all regions of Fallopian tube.
Specimen 1H: postnatal adult (more )
Note: ampulla region. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Structure Level Pattern Image Note
TS28: oviduct ampulla Present Regionally restricted 1H
TS28: ciliated columnar oviduct epithelium Strong Regionally restricted 1H In area of ampulla, expression clearly localized in basal layer of cilia of ciliated cells.
Specimen 1I: postnatal adult (more )
Note: isthmus region. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Structure Level Pattern Image Note
TS28: oviduct isthmus Strong Regionally restricted 1I High level of expression in epithelium of isthmus.
Specimen 1i: postnatal adult (more )
Note: Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Structure Level Pattern Image Note
TS28: epithelial layer of oviduct Present Regionally restricted 1i Expression accumulated more in apical cell cortx than in basal.
Specimen not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: endometrium Weak Expression in endometrium of uterus at markedly lower level.
Specimen 2A: postnatal adult (more )
Note: partial rim stage. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 2A When chromatic starts to condense as a partial rim around the nucleolus, expression localized to germinal vesicle.
Specimen 2a: postnatal adult (more )
Note: Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte Present Diffuse 2a Diffuse expression across germinal vesicle.
Specimen 2B: postnatal adult (more )
Note: full rim stage. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte Present Spotted 2B Expression accumulated in spots distributed around condensed chromatin in germinal vesicle.
Specimen 2b: postnatal adult (more )
Note: Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 2b Some foci of expression attached to nucleolus.
Specimen 2C: postnatal adult (more )
Note: disappearance of nucleolus. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 2C When nuclear lamina and nucleolus start to disappear, expression remains in vicinity of chromosomes.
Specimen 2c: postnatal adult (more )
Note: Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte Present Spotted 2c Spots of expression in close vicinity of chromosomes.
Specimen 2E: postnatal adult (more )
Note: metaphase of meiosis I. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 2E With organization of chromosomes to the metaphase plate, expression distributed along metaphase spindle, colocalized with tubulin.
Specimen 2G: postnatal adult (more )
Note: anaphase of meiosis I. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 2G During chromosome separation, expression remained in vicinity of chromosomes, locallized with meiotic spindle, colocalized with tubulin.
Specimen 2I: postnatal adult (more )
Note: metaphase of meiosis II. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Structure Level Pattern Image Note
TS28: secondary oocyte Present Regionally restricted 2I With organization of chromosomes to the metaphase plate, distributed along metaphase spindle, colocalized with tubulin.
Specimen 2K: postnatal adult (more )
Note: telophase of meiosis II. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Structure Level Pattern Image Note
TS28: secondary oocyte Present Regionally restricted 2K During final steps of meiosis II, expression located in meiotic spindle, colocalized with tubulin.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory