Antibody:	Anti-Nwd1
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: diencephalon ventricular layer	Present		4c
TS21: hindbrain ventricular layer	Present		4c
TS21: telencephalon ventricular layer	Present		4c
TS21: cortical plate	Present	Regionally restricted	4c , 4e	Expression was detected in migrating postmitotic neurons.

Structure	Level	Pattern	Image	Note
TS21: ganglionic eminence	Present	Regionally restricted	4d	Expression was detected in the ventricular zone.
TS21: spinal cord ventricular layer	Present		4d
TS21: trigeminal ganglion	Present		4d

Structure	Level	Pattern	Image	Note
TS21: dorsal root ganglion	Present		4g

Structure	Level	Pattern	Image	Note
TS21: sympathetic ganglion	Present		4h

Structure	Cell Type	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	neuron	Weak		5a' , 5a	Lower but detectable expression was observed in immature neurons migrating through the intermediate zone and accumulating in the cortical plate.
TS24: cortical plate	neuron	Weak		5a' , 5a	Lower but detectable expression was observed in immature neurons migrating through the intermediate zone and accumulating in the cortical plate.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cerebral cortex intermediate zone	neuron	Weak		5b , 5b'	Lower but detectable expression was observed in immature neurons migrating through the intermediate zone and accumulating in the cortical plate.
TS27: cortical plate	neuron	Weak		5b , 5b'	Lower but detectable expression was observed in immature neurons migrating through the intermediate zone and accumulating in the cortical plate.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex		Present		5c , 5c'
TS28: cerebral cortex vascular element	endothelial cell	Present		5c' , 5c	Endothelial cells of the capillary blood vessels were labeled at P7.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	neuron	Present	Widespread	5d , 5d'	Elevated immunoreactivity was detected in the cytoplasm of most neocortical neurons.
TS28: cortical layer IV	cerebral cortex pyramidal neuron	Present		5d' , 5d
TS28: cortical layer V	cerebral cortex pyramidal neuron	Present		5d' , 5d

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neocortex	neuron	Present	Widespread	5e , 5e'	Elevated immunoreactivity was detected in the cytoplasm of most neocortical neurons.
TS28: cortical layer IV	cerebral cortex pyramidal neuron	Present		5e , 5e'
TS28: cortical layer V	cerebral cortex pyramidal neuron	Present		5e , 5e'

Structure	Cell Type	Level	Pattern	Image	Note
TS28: corpus callosum		Weak	Single cells	5f , 5g
TS28: neocortex	neuron	Present	Widespread	5f	Elevated immunoreactivity was detected in the cytoplasm of most neocortical neurons. Expression pattern was observed throughout the cerebral cortex regardless of the cortical area (somatosensory, motor, or entorhinal cortex).
TS28: cortical layer IV	cerebral cortex pyramidal neuron	Present		5f	Expression pattern was observed throughout the cerebral cortex regardless of the cortical area (somatosensory, motor, or entorhinal cortex).
TS28: cortical layer V	cerebral cortex pyramidal neuron	Present		5f	Expression pattern was observed throughout the cerebral cortex regardless of the cortical area (somatosensory, motor, or entorhinal cortex).
TS28: brain vascular element	endothelial cell	Weak		5f

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	cerebellar granule cell	Weak		6a , 6a'
TS28: cerebellum Purkinje cell layer	Purkinje cell	Weak		6a' , 6a
TS28: blood vessel		Weak		6a' , 6a

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	neuron	Present		6b , 6b'	Expression was detected in immature neurons.
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		6b , 6b'
TS28: cerebellum white matter		Present	Regionally restricted	6b	Many immunopositive small-sized cells with an elongated morphology were sparsely distributed in the folial white matter tracts. These are presumed immature glial cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		6c' , 6c
TS28: cerebellum internal granule cell layer	cerebellar granule cell	Trace		6c , 6c'
TS28: cerebellum molecular layer	interneuron	Weak		6c , 6c'
TS28: cerebellum white matter		Ambiguous		6c	Little or no expression was observed in the folial white matter of the adult.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: deep cerebellar nucleus	neuron	Present		6d	Expression was evident in the large projection neurons of the deep cerebellar nuclei.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Purkinje cell	Present		6e	Expression was observed at the perinuclear region of the cytoplasm.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Present		7a

Structure	Cell Type	Level	Pattern	Image	Note
TS28: hippocampus	hippocampal pyramidal neuron	Present		7b
TS28: brain vascular element	endothelial cell	Present		7b

Structure	Cell Type	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	hippocampal pyramidal neuron	Present		7c
TS28: CA2 pyramidal cell layer	hippocampal pyramidal neuron	Present		7c
TS28: CA3 pyramidal cell layer	hippocampal pyramidal neuron	Present		7c
TS28: dentate gyrus granule cell layer	dentate gyrus granule cell	Present		7c
TS28: dentate gyrus hilus	dentate gyrus of hippocampal formation basket cell	Present		7c
TS28: dentate gyrus hilus	interneuron	Present		7c

Structure	Cell Type	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	hippocampal pyramidal neuron	Present		7d

Structure	Cell Type	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	mitral cell	Present		8a
TS28: main olfactory bulb external plexiform layer	interneuron	Present		8a
TS28: main olfactory bulb external plexiform layer	olfactory bulb tufted cell	Present		8a
TS28: main olfactory bulb glomerular layer	periglomerular cell	Present		8a
TS28: main olfactory bulb granule cell layer	olfactory granule cell	Weak		8a

Structure	Cell Type	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	mitral cell	Present		8b
TS28: main olfactory bulb external plexiform layer	interneuron	Present		8b
TS28: main olfactory bulb external plexiform layer	olfactory bulb tufted cell	Present		8b
TS28: main olfactory bulb granule cell layer	olfactory granule cell	Weak		8b

Structure	Cell Type	Level	Pattern	Image	Note
TS28: facial nucleus	neuron	Strong		8d , 8c
TS28: trigeminal nucleus	neuron	Strong		8c
TS28: vestibular nucleus		Strong		8c
TS28: medullary reticular formation		Strong	Regionally restricted	8c	Strong immunoreactivity was noted in the linear nucleus of the medulla oblongata.

Structure	Level	Pattern	Image	Note
TS28: medial habenular nucleus	Strong		8e

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Strong		8f

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Strong		8g

Structure	Cell Type	Level	Pattern	Image	Note
TS28: amygdala	neuron	Strong		8h
TS28: basolateral amygdaloid nucleus	neuron	Strong		8h
TS28: lateral amygdaloid nucleus	neuron	Strong		8h

Structure	Cell Type	Level	Pattern	Image	Note
TS28: red nucleus	neuron	Present
TS28: substantia nigra pars compacta	neuron	Present