Antibody:	anti-ERbeta (503)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	1A	Expression specifically localized in wall of midbrain.

Structure	Level	Pattern	Image	Note
TS20: midbrain lateral wall	Strong		1B

Structure	Level	Pattern	Image	Note
TS20: midbrain lateral wall	Strong	Regionally restricted	1C	Clear and robust nuclear expression mainly on the outside.

Structure	Level	Pattern	Image	Note
TS20: neuromere	Present	Scattered	1D	Scattered expressing cells in neuromere.

Structure	Level	Pattern	Image	Note
TS20: vestibulo-cochlear ganglion complex	Present	Regionally restricted		Expression in wall of vestibulocochlear ganglion complex.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	1E	Nuclear expression in hypothalamus and thalamus.
TS20: thalamus	Present	Regionally restricted	1E	Nuclear expression in hypothalamus and thalamus.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	1F	Clear nuclear expression localized in hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: pons	Present	Regionally restricted	1G	Clear nuclear expression localized in basal plate of pons.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Strong		2A
TS23: thalamus	Strong		2A

Structure	Level	Pattern	Image	Note
TS23: hypothalamus	Strong		2B

Structure	Level	Pattern	Image	Note
TS23: corpus striatum	Present	Scattered	2C	Scattered nuclear expression in striatum

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb	Strong		2D	Very clear and strong expression in olfactory bulb.

Structure	Level	Pattern	Image	Note
TS23: amygdala	Present
TS23: hippocampus	Present

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	2E	Nuclear expression in deep layer of cerebral cortex, but not in superficial layer.

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Present	Scattered	2F	Expression in scattered nuclei in cerebellum.

Structure	Level	Pattern	Image	Note
TS24: brain	Strong	Widespread		Expression increased significantly in brain at E16.5. Strong expression widely distributed in brain.

Structure	Level	Pattern	Image	Note
TS26: thalamus	Strong	Regionally restricted	3A	Robust nuclear expression in thalamus.

Structure	Level	Pattern	Image	Note
TS26: hypothalamus	Strong	Regionally restricted	3B	Robust nuclear expression in hypothalamus.

Structure	Level	Pattern	Image	Note
TS26: midbrain lateral wall	Present		3C inset , 3C
TS26: pons	Present		3C inset , 3C
TS26: medulla oblongata	Present	Regionally restricted	3C inset , 3C	Clear nuclear expression in medulla oblongata.

Structure	Level	Pattern	Image	Note
TS26: neocortex	Present	Regionally restricted	3D , 3D inset	Clear nuclear expression in neocortex, located mainly is deep layer, but also scattered nuclear expression in upper layer.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present	Regionally restricted	3E , 3E inset	Clear nuclear expression in cerebellum

Structure	Level	Pattern	Image	Note
TS26: corpus striatum	Present	Regionally restricted	3F , 3F inset	Clear nuclear expression in striatum.

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb external plexiform layer	Present		3G inset , 3G	Expressing cells in mitral cell and plexiform layers.
TS26: main olfactory bulb internal plexiform layer	Present		3G inset , 3G	Expressing cells in mitral cell and plexiform layers.
TS26: main olfactory bulb mitral cell layer	Present		3G inset , 3G	Expressing cells in mitral cell and plexiform layers.

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Present		3H
TS26: hippocampus	Present	Regionally restricted	3H	Strong expression in pyramidal cellular layer of hippocampus.