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Gene Expression Data
Assay Details
Assay
Reference: J:133832 Festoff BW, et al., Motor neuron cell death in wobbler mutant mice follows overexpression of the G-protein-coupled, protease-activated receptor for thrombin. Mol Med. 2000 May;6(5):410-29
Assay type: RT-PCR
MGI Accession ID: MGI:6850702
Gene symbol: F2r
Gene name: coagulation factor II thrombin receptor
Probe: PAR1-pC, PAR1-pD
Visualized with: Ethidium bromide
Assay notes: Data from Table 3. Quantitative reaction using a mimic product (a subclone with a deletion introduced - pRMS11). Titrated amounts of control, mimic product into the RT reaction prior to amplification allowed the estimated copy number of transcript in the wr/wr spinal cord compared with wild type.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 660 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex
wt E12 E12.0 TS20: spinal cord Present 1 µg; total RNA involves: NFR/N Not Specified
wr/wr E12 E12.0 TS20: spinal cord Present 1 µg; total RNA involves: NFR/N Vps54wr/Vps54wrNot Specified
wt B P newborn TS27: spinal cord Present 1 µg; total RNA involves: NFR/N Not Specified
wr/wr B P newborn TS27: spinal cord Present 1 µg; total RNA involves: NFR/N Vps54wr/Vps54wrNot Specified
wt P5 P5 TS28: spinal cord Present 1 µg; total RNA involves: NFR/N Not Specified
wr/wr P5 P5 TS28: spinal cord Present 1 µg; total RNA involves: NFR/N Vps54wr/Vps54wrNot Specified
wt P18 P18 TS28: spinal cord Present 1 µg; total RNA involves: NFR/N Not Specified
wr/wr P18 P18 TS28: spinal cord Present 1 µg; total RNA involves: NFR/N Vps54wr/Vps54wrNot Specified
wt P28 P28 TS28: spinal cord Present 1 µg; total RNA involves: NFR/N Not Specified
wr/wr P28 P28 TS28: spinal cord Present 1 µg; total RNA involves: NFR/N Vps54wr/Vps54wrNot Specified
Notes:
(a) In control animals, expression was biphasic, with high to low levels in the embryonic spinal cord and a second peak during postnatal development and continued low levels in the adult. wr/wr mice display elevated expression as early as E12, which continued at this level into adulthood, with a possible further increase (8 fold) in the early postnatal period.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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