About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:133832 Festoff BW, et al., Motor neuron cell death in wobbler mutant mice follows overexpression of the G-protein-coupled, protease-activated receptor for thrombin. Mol Med. 2000 May;6(5):410-29
Assay type: RT-PCR
MGI Accession ID: MGI:6850701
Gene symbol: F2r
Gene name: coagulation factor II thrombin receptor
Probe: PAR1-pC, PAR1-pD
Visualized with: Ethidium bromide
Assay notes: Quantitative reaction using a mimic product (a subclone with a deletion introduced - pRMS11). Titrated amounts of control, mimic product into the RT reaction prior to amplification allowed the estimated copy number of transcript in the wr/wr spinal cord compared with wild type.
Results Image: 9
 Sample Information Bands Other Sample Information
Lane AgeStructure 660 bp Amount Genetic BackgroundMutant Allele(s)Sex
Marker Mol. Wt. Marker Lane
No template Control
Mimic RNA only Control
Par-1 cDNA only E12.0 TS20: spinal cord Present 1 µg; total RNA involves: NFR/N \Vps54wr/\Vps54wrNot Specified
1 ug wr + 20 fg mimic E12.0 TS20: spinal cord Present 1 µg; total RNA involves: NFR/N \Vps54wr/\Vps54wrNot Specified
1 ug wr + 10 fg mimic E12.0 TS20: spinal cord Present 1 µg; total RNA involves: NFR/N \Vps54wr/\Vps54wrNot Specified

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/25/2025
MGI 6.24
The Jackson Laboratory