Gene Expression Data
Assay Details
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Assay
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Reference:
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J:133832
Festoff BW, et al., Motor neuron cell death in wobbler mutant mice follows overexpression of the G-protein-coupled, protease-activated receptor for thrombin. Mol Med. 2000 May;6(5):410-29
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6850685
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Gene symbol:
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F2r
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Gene name:
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coagulation factor II thrombin receptor
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Antibody:
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PAR-1C
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3A:
postnatal day 28
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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involves: NFR/N
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Age:
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postnatal day 28
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3B:
postnatal day 28
(more )
Specimen
3B:
(close )
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Genetic Background:
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involves: NFR/N
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Age:
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postnatal day 28
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spinal cord ventral horn |
Present |
Regionally restricted |
3B
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Large ventral horn motor neurons were primarily labeled. Faint staining was observed on smaller interneurons.
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| TS28: spinal cord dorsal horn |
Weak |
Regionally restricted |
3B
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Faint staining was observed over dorsal horn neurons.
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Specimen
3C:
(close )
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Genetic Background:
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involves: NFR/N
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Mutant Allele(s):
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\Vps54wr/\Vps54wr
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Age:
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postnatal day 28
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3D:
(close )
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Genetic Background:
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involves: NFR/N
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Mutant Allele(s):
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\Vps54wr/\Vps54wr
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Age:
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postnatal day 28
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spinal cord ventral horn |
Strong |
Regionally restricted |
3D
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Staining in the thoracolumbar spinal cord sections was similar to the intensity range of controls, where a number of large neuronal profiles consistent with motoneurons were not stained.
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| TS28: spinal cord dorsal horn |
Strong |
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3D
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Expression was increased in the dorsal horn of the mutant compared to wild type.
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Specimen
not shown:
postnatal day 28
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: NFR/N
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Age:
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postnatal day 28
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown:
(close )
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Genetic Background:
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involves: NFR/N
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Mutant Allele(s):
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\Vps54wr/\Vps54wr
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Age:
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postnatal day 28
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spinal cord lumbar region |
Present |
Regionally restricted |
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Staining in the thoracolumbar spinal cord sections was similar to the intensity range of controls, where a number of large neuronal profiles consistent with motoneurons were not stained.
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| TS28: spinal cord thoracic region |
Present |
Regionally restricted |
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Staining in the thoracolumbar spinal cord sections was similar to the intensity range of controls, where a number of large neuronal profiles consistent with motoneurons were not stained.
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Analysis Tools