About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:308859 Akter MS, et al., CRISPR/Cas9-based genetic screen of SCNT-reprogramming resistant genes identifies critical genes for male germ cell development in mice. Sci Rep. 2021 Jul 29;11(1):15438
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6850196
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: Anti-DDX4/MVH (ab13840)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 2e Control: postnatal week 12-15 (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Single cells 2e Control
Specimen 2e sgFaiml: postnatal week 12-15; Faimlem1Smtb/Faimlem1Smtb (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Single cells 2e sqFaiml
Specimen 2e sgCox8c: postnatal week 12-15; Cox8cem1Smtb/Cox8cem1Smtb (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Single cells 2e sqCox8c
Specimen 2e sgCox7b2: postnatal week 12-15; Cox7b2em1Smtb/Cox7b2em1Smtb (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Single cells 2e sqCox7b2
Specimen 2e sgGm773: postnatal week 12-15; Gm773em1Smtb/Y (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Single cells 2e sqGm773
Specimen 2e sgMajin-2: postnatal week 12-15; Majinem1Smtb/Majinem1Smtb (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Single cells 2e sqMajin-2 Germ cells are present but arrested in meiosis in this mutant.
Specimen 2e sgMajin-3: postnatal week 12-15; Majinem2Smtb/Majinem2Smtb (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Single cells 2e sqMajin-3 Germ cells are present but arrested in meiosis in this mutant.
Specimen 2e sgTuba3b: postnatal week 12-15; Tuba3bem1Smtb/Tuba3bem1Smtb (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Single cells 2e sqTuba3b
Specimen 2e sgTuba3a: postnatal week 12-15; Tuba3aem1Smtb/Tuba3aem1Smtb (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Single cells 2e sqTuba3a
Specimen 2e sgTuba3a/3b: postnatal week 12-15; Tuba3aem2Smtb/Tuba3aem2Smtb, Tuba3bem2Smtb/Tuba3bem2Smtb (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Absent 2e sqTuba3a/3b
Specimen 6a Control E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: germ cell of testis Present Single cells 6a Control E16.5
Specimen 6a sgTuba3a/3b E16.5: embryonic day 16.5; Tuba3aem2Smtb/Tuba3aem2Smtb, Tuba3bem2Smtb/Tuba3bem2Smtb (more )
Structure Level Pattern Image Note
TS24: germ cell of testis Present Single cells 6a sgTuba3a/3b E16.5 The number of germ cells present is similar in mutant and wild type.
Specimen 6a Control E19.5: postnatal day 0 (more )
Note: The embryos were recovered at E19.5 by caesarian section.
Structure Level Pattern Image Note
TS27: germ cell of testis Present Single cells 6a Control E19.5
Specimen 6a sgTuba3a/3b E19.5: postnatal day 0; Tuba3aem2Smtb/Tuba3aem2Smtb, Tuba3bem2Smtb/Tuba3bem2Smtb (more )
Note: The embryos were recovered at E19.5 by caesarian section.
Structure Level Pattern Image Note
TS27: germ cell of testis Present Single cells 6a sgTuba3a/3b E19.5 The number of germ cells present in the mutant is slightly reduced compared to the number seen in wild type.
Specimen 6a Control P3: postnatal day 3 (more )
Structure Level Pattern Image Note
TS27: germ cell of testis Present Single cells 6a Control P3
Specimen 6a sgTuba3a/3b P3: postnatal day 3; Tuba3aem2Smtb/Tuba3aem2Smtb, Tuba3bem2Smtb/Tuba3bem2Smtb (more )
Structure Level Pattern Image Note
TS27: germ cell of testis Present Single cells 6a sgTuba3a/3b P3 The number of germ cells present in the mutant is reduced relative to the number seen in wild type.
Specimen 6a Control P7: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Single cells 6a Control P7
Specimen 6a sgTuba3a/3b P7: postnatal day 7; Tuba3aem2Smtb/Tuba3aem2Smtb, Tuba3bem2Smtb/Tuba3bem2Smtb (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Single cells 6a sgTuba3a/3b P7 The number of germ cells present in the mutant is reduced relative to the number seen in wild type.
Specimen 6a Control P21: postnatal day 21 (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Single cells 6a Control P21
Specimen 6a sgTuba3a/3b P21: postnatal day 21; Tuba3aem2Smtb/Tuba3aem2Smtb, Tuba3bem2Smtb/Tuba3bem2Smtb (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Absent 6a sgTuba3a/3b P21
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
01/28/2026
MGI 6.24 		The Jackson Laboratory