H2-M3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:225528 Liu J, et al., Spatial-Temporal Expression of Non-classical MHC Class I Molecules in the C57 Mouse Brain. Neurochem Res. 2015 Jul;40(7):1487-96
Assay type:	RNA in situ
MGI Accession ID:	MGI:6850071
Gene symbol:	H2-M3
Gene name:	histocompatibility 2, M region locus 3

Probe:	M3 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 1A: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		1A
TS20: allocortex	Present	Regionally restricted	1A	Extensive expression in hippocampus neuroepithelium.
TS20: lateral ventricle	Present	Regionally restricted	1A	Extensive expression in neuroepithelium of lateral ventricle.

Specimen 1B: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: mesencephalic vesicle	Present	Regionally restricted	1B	Expression in neuroepithelium of aqueduct.

Specimen 1C: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present	Regionally restricted	1C	Expression in neuroepithelium of cerebellum.

Specimen 1E: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present		1E
TS24: hippocampus	Present	Regionally restricted	1E	Extensive expression in hippocampus neuroepithelium.
TS24: lateral ventricle	Present	Regionally restricted	1E	Extensive expression in neuroepithelium of lateral ventricle.

Specimen 1F: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present		1F
TS24: hippocampus	Present	Regionally restricted	1F	Extensive expression in hippocampus neuroepithelium.
TS24: brain commissure	Present	Regionally restricted	1F	Expression in habenular commissure.

Specimen 1G: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebral aqueduct	Present	Regionally restricted	1G	Expression in neuroepithelium of aqueduct.

Specimen 1H: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Present	Regionally restricted	1H	Expression in neuroepithelium of cerebellum.

Specimen 2A: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb glomerular layer	Present		2A	Prominent expression in mitral cell layer and glomerular layer of olfactory bulb from P0 to adult.
TS27: main olfactory bulb mitral cell layer	Present		2A	Prominent expression in mitral cell layer and glomerular layer of olfactory bulb from P0 to adult.

Specimen 2B: postnatal day 4 (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		2B	Expression in mitral cell layer and glomerular layer of olfactory bulb gradually increased from P0 to P15.
TS28: main olfactory bulb mitral cell layer	Present		2B	Expression in mitral cell layer and glomerular layer of olfactory bulb gradually increased from P0 to P15.

Specimen 2C: postnatal day 8 (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		2C	Expression in mitral cell layer and glomerular layer of olfactory bulb gradually increased from P0 to P15.
TS28: main olfactory bulb mitral cell layer	Present		2C	Expression in mitral cell layer and glomerular layer of olfactory bulb gradually increased from P0 to P15.

Specimen 2D: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong		2D	Expression in mitral cell layer and glomerular layer of olfactory bulb gradually increased from P0 to P15.
TS28: main olfactory bulb mitral cell layer	Strong		2D	Expression in mitral cell layer and glomerular layer of olfactory bulb gradually increased from P0 to P15.

Specimen 2E: postnatal day 30 (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		2E
TS28: main olfactory bulb mitral cell layer	Present		2E

Specimen 2F: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Weak		2F	Expression in mitral cell layer and glomerular layer of olfactory bulb decreased from P30 to adult.
TS28: main olfactory bulb mitral cell layer	Weak		2F	Expression in mitral cell layer and glomerular layer of olfactory bulb decreased from P30 to adult.

Specimen 3A: postnatal day 0 (more

)

Structure	Level	Image
TS27: dentate gyrus	Present	3A
TS27: dorsal lateral geniculate nucleus	Present	3A
TS27: hippocampus	Present	3A

Specimen 3B: postnatal day 4 (more

)

Structure	Level	Image
TS28: dentate gyrus	Present	3B
TS28: dorsal lateral geniculate nucleus	Present	3B
TS28: hippocampus	Present	3B

Specimen 3C: postnatal day 8 (more

)

Structure	Level	Image
TS28: dentate gyrus	Present	3C
TS28: dorsal lateral geniculate nucleus	Present	3C
TS28: hippocampus	Present	3C

Specimen 3D: postnatal day 15 (more

)

Structure	Level	Image
TS28: dentate gyrus	Present	3D
TS28: dorsal lateral geniculate nucleus	Present	3D
TS28: hippocampus	Present	3D

Specimen 3E: postnatal day 30 (more

)

Structure	Level	Image	Note
TS28: dentate gyrus	Weak	3E	Weaker expression cornu ammonis, dentate gyrus and dorsal-lateral geniculate nucleus at P30 than at P15.
TS28: dorsal lateral geniculate nucleus	Weak	3E	Weaker expression cornu ammonis, dentate gyrus and dorsal-lateral geniculate nucleus at P30 than at P15.
TS28: hippocampus	Weak	3E	Weaker expression cornu ammonis, dentate gyrus and dorsal-lateral geniculate nucleus at P30 than at P15.

Specimen 3F: postnatal adult (more

)

Structure	Level	Image
TS28: dentate gyrus	Absent	3F
TS28: dorsal lateral geniculate nucleus	Absent	3F
TS28: hippocampus	Absent	3F

Specimen 4A: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebellar cortex	Present		4A	Expression in cerebellum from P0 to P30. Expression mainly in cerebellar cortex layer from P0 to P4.

Specimen 4B: postnatal day 4 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellar cortex	Present		4B	Expression mainly in cerebellar cortex layer from P0 to P4.
TS28: cerebellum Purkinje cell layer	Present		4B	Expression in Purkinje cell layer from P4 to P30.

Specimen 4C: postnatal day 8 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4C	Expression in Purkinje cell layer from P4 to P30.

Specimen 4D: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4D	Expression in Purkinje cell layer from P4 to P30.

Specimen 4E: postnatal day 30 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4E	Expression in Purkinje cell layer from P4 to P30.

Specimen 4F: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Absent		4F

Specimen P4 not shown: postnatal day 4 (more

)

Structure	Level	Pattern	Image	Note
TS28: brain nucleus	Present			Expression in some nerve nuclei begins to appear at P4.

Specimen 5A: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: brain commissure	Present	Regionally restricted	5A	Expression in habenular commissure. Peak expression at P15.

Specimen 5B: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: locus coeruleus	Present		5B	Peak expression at P15.

Specimen 5C: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: cochlear nucleus	Present		5C	Peak expression at P15.

Specimen 5D: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: medial vestibular nucleus	Present		5D	Peak expression at P15.

Specimen 5E: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: gigantocellular reticular nucleus	Present		5E	Peak expression at P15.

Specimen 5F: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: hypoglossal nucleus	Present		5F	Peak expression at P15.

Specimen 5G: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Present		5G	Peak expression at P15.

Specimen S2A: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		S2A
TS28: main olfactory bulb mitral cell layer	Present		S2A

Specimen S2B: postnatal day 15 (more

)

Structure	Level	Image
TS28: dentate gyrus	Present	S2B
TS28: dorsal lateral geniculate nucleus	Present	S2B
TS28: hippocampus	Present	S2B

Specimen S2C: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		S2C

Specimen S3A: postnatal day 0; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb glomerular layer	Present		S3A	Expression in mitral cell layer and glomerular layer of olfactory bulb from P0 to P15 paralled that of wild-type mice.
TS27: main olfactory bulb mitral cell layer	Present		S3A	Expression in mitral cell layer and glomerular layer of olfactory bulb from P0 to P15 paralled that of wild-type mice.

Specimen S3B: postnatal day 4; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		S3B	Expression in mitral cell layer and glomerular layer of olfactory bulb from P0 to P15 paralled that of wild-type mice.
TS28: main olfactory bulb mitral cell layer	Present		S3B	Expression in mitral cell layer and glomerular layer of olfactory bulb from P0 to P15 paralled that of wild-type mice.

Specimen S3C: postnatal day 8; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		S3C
TS28: main olfactory bulb mitral cell layer	Present		S3C

Specimen S3D: postnatal day 8 (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Weak		S3D
TS28: main olfactory bulb mitral cell layer	Weak		S3D

Specimen S3E: postnatal day 15; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Strong		S3E	Expression in mitral cell layer and glomerular layer of olfactory bulb. Higher expression in olfactory bulb of mutant than in wild-type at P8 and P15.
TS28: main olfactory bulb mitral cell layer	Strong		S3E	Expression in mitral cell layer and glomerular layer of olfactory bulb. Higher expression in olfactory bulb of mutant than in wild-type at P8 and P15.

Specimen S3F: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Weak		S3F
TS28: main olfactory bulb mitral cell layer	Weak		S3F

Specimen S4A: postnatal day 0; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Strong		S4A	Expression in Purkinje cell layer of cerebellum from P0 to P15.

Specimen S4B: postnatal day 4; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		S4B	Expression in Purkinje cell layer of cerebellum from P0 to P15.

Specimen S4C: postnatal day 8; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		S4C	Expression in Purkinje cell layer of cerebellum from P0 to P15.

Specimen S4D: postnatal day 15; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		S4D	Expression in Purkinje cell layer of cerebellum from P0 to P15.

Specimen S4E: postnatal day 15; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Strong		S4E	Higher expression in mutant compared to wild-type.

Specimen S4F: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Weak		S4F

Specimen 6A: postnatal day 0; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Image	Note
TS27: dentate gyrus	Trace	6A	Higher expression in mutant hippocampus than in wild-type.
TS27: dorsal lateral geniculate nucleus	Trace	6A	Higher expression in mutant hippocampus than in wild-type.
TS27: hippocampus	Trace	6A	Higher expression in mutant hippocampus than in wild-type.

Specimen 6B: postnatal day 0 (more

)

Structure	Level	Image
TS27: dentate gyrus	Trace	6B
TS27: dorsal lateral geniculate nucleus	Trace	6B
TS27: hippocampus	Trace	6B

Specimen 6D: postnatal day 4; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Image	Note
TS28: dentate gyrus	Present	6D	Higher expression in mutant hippocampus than in wild-type. Expression in hippocampus gradually increased from P0 to P15.
TS28: dorsal lateral geniculate nucleus	Present	6D	Higher expression in mutant hippocampus than in wild-type. Expression in hippocampus gradually increased from P0 to P15.
TS28: facial nucleus	Present	6D	Higher expression in mutant hippocampus than in wild-type. Expression in hippocampus gradually increased from P0 to P15.

Specimen 6E: postnatal day 4 (more

)

Structure	Level	Image
TS28: dentate gyrus	Trace	6E
TS28: dorsal lateral geniculate nucleus	Trace	6E
TS28: hippocampus	Trace	6E

Specimen 6G: postnatal day 8; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Image	Note
TS28: dentate gyrus	Strong	6G	Higher expression in mutant hippocampus than in wild-type. Expression in hippocampus gradually increased from P0 to P15.
TS28: dorsal lateral geniculate nucleus	Strong	6G	Higher expression in mutant hippocampus than in wild-type. Expression in hippocampus gradually increased from P0 to P15.
TS28: hippocampus	Strong	6G	Higher expression in mutant hippocampus than in wild-type. Expression in hippocampus gradually increased from P0 to P15.

Specimen 6H: postnatal day 8 (more

)

Structure	Level	Image	Note
TS28: dentate gyrus	Trace	6H	Higher expression in mutant hippocampus than in wild-type. Expression in hippocampus gradually increased from P0 to P15.
TS28: dorsal lateral geniculate nucleus	Trace	6H	Higher expression in mutant hippocampus than in wild-type. Expression in hippocampus gradually increased from P0 to P15.
TS28: hippocampus	Trace	6H	Higher expression in mutant hippocampus than in wild-type. Expression in hippocampus gradually increased from P0 to P15.

Specimen 6J: postnatal day 15; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: dorsal lateral geniculate nucleus	Very strong		6J	Higher expression in mutant hippocampus than in wild-type. Expression in hippocampus gradually increased from P0 to P15.
TS28: hippocampus	Very strong		6J	Higher expression in mutant hippocampus than in wild-type. Expression in hippocampus gradually increased from P0 to P15.

Specimen 6K: postnatal day 15 (more

)

Structure	Level	Image
TS28: dentate gyrus	Present	6K
TS28: dorsal lateral geniculate nucleus	Present	6K
TS28: hippocampus	Present	6K

Specimen 7A: postnatal day 4; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Strong		7A	Significantly increased expression in facial nucleus compared to wild type from P5 to P15.

Specimen 7B: postnatal day 8; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Strong		7B	Significantly increased expression in facial nucleus compared to wild type from P5 to P15.

Specimen 7C: postnatal day 15; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Strong		7C

Specimen 7D: postnatal day 4 (more

)

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Weak		7D

Specimen 7E: postnatal day 8 (more

)

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Weak		7E

Specimen 7F: postnatal day 15 (more

)

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Weak		7F

Specimen S5A: postnatal day 0; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS27: periaqueductal grey	Present		S5A	Expression in periaqueductal gray of midbrain aqueduct from P0 to P8.

Specimen S5B: postnatal day 0 (more

)

Structure	Level	Pattern	Image	Note
TS27: periaqueductal grey	Weak		S5B	Much lower expression in periaqueductal gray of wild type mice compared to mutant.

Specimen S5C: postnatal day 4; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: periaqueductal grey	Present		S5C	Expression in periaqueductal gray of midbrain aqueduct form P0 to P8.

Specimen S5D: postnatal day 8; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: periaqueductal grey	Present		S5D	Expression in periaqueductal gray of midbrain aqueduct form P0 to P8.

Specimen S5E: postnatal day 15; H2-D1^b-tm1Bpe/H2-D1^b-tm1Bpe, H2-K1^b-tm1Bpe/H2-K1^b-tm1Bpe (more

)

Structure	Level	Pattern	Image	Note
TS28: periaqueductal grey	Absent		S5E	No expression at P15.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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