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Gene Expression Data
Assay Details
Assay
Reference: J:308692 Anand D, et al., Genome-Wide Analysis of Differentially Expressed miRNAs and Their Associated Regulatory Networks in Lenses Deficient for the Congenital Cataract-Linked Tudor Domain Containing Protein TDRD7. Front Cell Dev Biol. 2021;9:615761
Assay type: RT-PCR
MGI Accession ID: MGI:6845097
Gene symbol: Mir15a
Gene name: microRNA 15a
Probe: miR-15a-pA, miR-15a-pB
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR was used to obtain these results. Mir17, which exhibits robust expression in the lens and whose expression level is not altered in the mutant lens, and the housekeeping genes Gapdh and Actb were used to normalize expression levels.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
Control P4 TS28: lens Present Not Specified; total RNA involves: 129S6/SvEvTac * C57BL/6J Not Specified littermate control; the genotype is either +/+ or +/-
Tdrd7-/- P4 TS28: lens Present (a) Not Specified; total RNA involves: 129S6/SvEvTac * C57BL/6J Tdrd7tm1.1Chum/Tdrd7tm1.1ChumNot Specified
Notes:
(a) Expression levels are significantly increased in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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