Probe:	Ntn3 cDNA4
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS15: future spinal cord	Absent		4B
TS15: dorsal root ganglion	Strong		4B	Highest expression in dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Absent		4F
TS17: dorsal root ganglion	Strong		4F	Highest expression in dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Weak	Regionally restricted	4J	Low levels of expression in motor column in ventral spinal cord.
TS19: neural tube floor plate	Absent		4J	No expression in floor plate or in ventricular zone.
TS19: neural tube ventricular layer	Absent		4J	No expression in floor plate or in ventricular zone.
TS19: dorsal root ganglion	Present		4J

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Absent		5A
TS17: dorsal root ganglion	Present		5A
TS17: embryo mesenchyme	Weak	Diffuse	5A	Low diffuse expression in mesenchyme at E10.5.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Weak	Regionally restricted	5B	Low level of expression in ventral spinal cord.
TS19: dorsal root ganglion	Present		5B
TS19: embryo mesenchyme	Present	Regionally restricted	5B	Expression in mesenchyme mostly localized in condensing mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Weak	Regionally restricted	5C	Low level of expression in ventral spinal cord.
TS20: dorsal root ganglion	Present		5C	Expression in dorsal root ganglion persists through E12.5.
TS20: limb mesenchyme	Present	Regionally restricted	5C	Expression in mesenchyme mostly localized in condensing mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Weak	Regionally restricted	5D	Low level of expression in ventral spinal cord through E14.5.
TS22: dorsal root ganglion	Weak		5D	Expresison in dorsal root ganglia reduced in intensity by E14.5
TS22: skeletal muscle	Present	Regionally restricted	5D	Expression in differentiating myotubes. Expression in muscles similar to that of Ntn1

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Present	Regionally restricted	5E	Expression in differentiating myotubes. Expression in muscles similar to that of Ntn1
TS22: lung	Present		5E	Expression in lung similar to that of Ntn1.

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Present		5F	Expression in differentiating myotubes. Expression in muscles similar to that of Ntn1

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		6A	In head region, expression highest in differentiating trigeminal (V) and geniculate (VII) ganglia.
TS15: trigeminal preganglion	Present		6A	In head region, expression highest in differentiating trigeminal (V) and geniculate (VII) ganglia.

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present		6B	Expression in trigeminal (V), geniculate (VII), acoustic (VIII) ganglia.
TS17: facial ganglion	Present		6B	Expression in trigeminal (V), geniculate (VII), acoustic (VIII) ganglia.
TS17: trigeminal ganglion	Present		6B	Expression in trigeminal (V), geniculate (VII), acoustic (VIII) ganglia.

Structure	Level	Pattern	Image	Note
TS19: trigeminal ganglion	Present		6C	Expression in trigeminal (V) ganglia.
TS19: diencephalon	Present	Regionally restricted	6C	Expression in developing thalamus.
TS19: embryo mesenchyme	Weak		6C	Lower levels of expression in mesenchymal tissues.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Weak	Regionally restricted	6D	Low level of expression in ventral spinal cord.
TS20: glossopharyngeal ganglion	Present		6D	Expression in trigeminal (V), glossopharyngeal (IX), nodose (X) and jugular (X) ganglia persists through E12.5.
TS20: inferior vagus ganglion	Present		6D	Expression in trigeminal (V), glossopharyngeal (IX), nodose (X) and jugular (X) ganglia persists through E12.5.
TS20: superior vagus ganglion	Present		6D	Expression in trigeminal (V), glossopharyngeal (IX), nodose (X) and jugular (X) ganglia persists through E12.5.
TS20: trigeminal ganglion	Present		6D	Expression in trigeminal (V), glossopharyngeal (IX), nodose (X) and jugular (X) ganglia persists through E12.5.
TS20: thalamus	Present		6D	Expression in developing thalamus.
TS20: embryo mesenchyme	Weak		6D	Lower levels of expression in mesenchymal tissues.

Structure	Level	Pattern	Image	Note
TS22: cranial ganglion	Weak			Expression reduced by E14.5.

Structure	Level	Pattern	Image	Note
TS24: brain	Weak	Diffuse		Moderate or low levels of very diffuse expression in many levels of brain.