Probe:	Mm-Nrgn (499441)
Probe preparation:	Antisense
Assay notes:	RNAscope assay.

Structure	Level	Pattern	Image	Note
TS26: brain	Present		3B +/+
TS26: frontal cortex	Present		3B +/+ , 3C +/+ , 3D +/+ upper , 3D +/+ lower	Expression is seen in the motor and frontal/cingular cortex.
TS26: piriform cortex	Present		3E +/+ , 3B +/+
TS26: corpus striatum	Present		3F +/+ , 3E +/+ , 3B +/+ , 3G +/+
TS26: parietal cortex	Present		3B +/+ , 3G +/+	Expression is seen in the second somatosensory cortex.

Structure	Level	Pattern	Image	Note
TS26: brain	Present		3B -/-	Expression levels are increased in most brain regions in the mutant. Increases are most dramatic in the frontal/cingular cortex and the motor cortex.
TS26: frontal cortex	Present		3D -/- upper , 3C -/- , 3B -/- , 3D -/- lower	Expression is seen in the motor and frontal/cingular cortex; its level is increased in the mutant.
TS26: piriform cortex	Present		3E -/- , 3B -/-	Expression levels are comparable to those seen in wild type.
TS26: corpus striatum	Present		3E -/- , 3B -/- , 3F -/- , 3G -/-	Expression levels are increased in the mutant.
TS26: parietal cortex	Present		3G -/- , 3B -/-	Expression is seen in the second somatosensory cortex; its level is increased in the mutant.

Structure	Level	Pattern	Image	Note
TS26: brain	Present		S2A +/+	No expression is noted in the outermost layer of the cortex.
TS26: temporal lobe	Present		S2B +/+ top , S2A +/+	Expression is seen in the temporal cortex.
TS26: parietal cortex	Present		S2B +/+ top , S2A +/+ , S2B +/+ center
TS26: corpus striatum	Present		S2B +/+ top , S2A +/+
TS26: frontal cortex	Present		S2B +/+ bottom , S2A +/+	Expression is seen in the cingular/prefrontal cortex.

Structure	Level	Pattern	Image	Note
TS26: brain	Present		S2A -/-	Expression levels are increased in most brain regions in the mutant. Increases are most dramatic in the frontal/cingular cortex and the motor cortex. As in wild type, no expression is noted in the outermost layer of the cortex.
TS26: temporal lobe	Present		S2A -/- , S2B -/- top	Expression is seen in the temporal cortex; its level is increased in the mutant.
TS26: parietal cortex	Present		S2B -/- center , S2A -/- , S2B -/- top	Expression levels are increased in the mutant.
TS26: corpus striatum	Present		S2B -/- top , S2A -/-	Expression levels are increased in the mutant.
TS26: frontal cortex	Present		S2B -/- bottom , S2A -/-	Expression is seen in the cingular/prefrontal cortex; its level is increased in the mutant.