Tg(Prrx1-Shh*)NPtch
Transgene Detail
|
|
| Symbol: |
Tg(Prrx1-Shh*)NPtch |
| Name: |
transgene insertion N, Pao-Tien Chuang |
| MGI ID: |
MGI:6836755 |
| Transgene: |
Tg(Prrx1-Shh*)NPtch Location: unknown
|
| Alliance: |
Tg(Prrx1-Shh*)NPtch page
|
|
| Strain of Origin: |
Not Applicable
|
|
| Transgene Type: |
|
Transgenic (Inserted expressed sequence) |
| Mutation: |
|
Insertion
|
| |
|
Tg(Prrx1-Shh*)NPtch expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Shh (MGI:98297) |
"mouse enhancer, modified mouse cDNA" |
|
| |
|
Mutation details: A mouse sonic hedgehog cDNA lacking the sequence for the cholesterol attachment and with a p.C25S mutation (to prevent palmitoylation of the peptide) was placed under the control of the mouse paired related homeobox 1 enhancer to drive its expression in embryonic limb buds. The NonCholesterol Modified mutation involves inserting a loxP site flanked neomycin resistance gene cassette into the coding region in exon 3 upstream of the cholesterylation site, which results in a premature stop codon after glycine codon 198. The neo cassette was removed through subsequent cre-mediated recombination.
(J:89228)
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
|
| Original: |
J:89228 Chen MH, et al., Palmitoylation is required for the production of a soluble multimeric Hedgehog protein complex and long-range signaling in vertebrates. Genes Dev. 2004 Mar 15;18(6):641-59 |
| All: |
1 reference(s) |
|
Analysis Tools
