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Gene Expression Data
Assay Details
Assay
Reference: J:226326 Greenlees R, et al., Mutations in SIPA1L3 cause eye defects through disruption of cell polarity and cytoskeleton organization. Hum Mol Genet. 2015 Oct 15;24(20):5789-804
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6828639
Gene symbol: Tjp1
Gene name: tight junction protein 1
Antibody: Anti-ZO-1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 6I: embryonic day 18.5 (more )
Note: Double labeled: green - Tjp1; red - F-actin.
Structure Level Pattern Image Note
TS26: lens anterior epithelium Present 6I Organized arrangement of the anterior monolayer was seen.
Specimen 6J: embryonic day 18.5; Sipa1l3Gt(EUCE0020a11)Hmgu/Sipa1l3Gt(EUCE0020a11)Hmgu (more )
Note: Double labeled: green - Tjp1; red - F-actin.
Structure Level Pattern Image Note
TS26: lens anterior epithelium Present 6J Staining continuity was disrupted, with disorganized arrangement of cells.
Specimen 6K: embryonic day 18.5 (more )
Note: Double labeled: green - Tjp1; red - F-actin.
Structure Level Pattern Image Note
TS26: anterior lens fiber Present 6K Typical staining and normal transition into the fibre cell layer were seen.
TS26: lens anterior epithelium Present 6K Typical staining and normal transition into the fibre cell layer were seen.
Specimen 6L: embryonic day 18.5; Sipa1l3Gt(EUCE0020a11)Hmgu/Sipa1l3Gt(EUCE0020a11)Hmgu (more )
Note: Double labeled: green - Tjp1; red - F-actin.
Structure Level Pattern Image Note
TS26: lens anterior epithelium Present Regionally restricted 6L Marked reduction of staining in the lens equatorial region.
TS26: anterior lens fiber Present 6L Expression in the apical tips of the dislocated fibre cells appeared to lack uniformity compared with the normal lens.
Specimen S4M: postnatal day 10 (more )
Note: Double labeled: green - Tjp1; red - F-actin.
Structure Level Pattern Image Note
TS28: lens anterior epithelium Present S4M
TS28: lens fiber Present S4M
Specimen S4N: postnatal day 10; Sipa1l3Gt(EUCE0020a11)Hmgu/Sipa1l3Gt(EUCE0020a11)Hmgu (more )
Note: Double labeled: green - Tjp1; red - F-actin.
Structure Level Pattern Image Note
TS28: lens anterior epithelium Present Regionally restricted S4N Loss of staining in the apical region.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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