Gene Expression Data
Assay Details
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Assay
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Reference:
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J:46239
Beck ARP, et al., RNA-binding protein TIAR is essential for primordial germ cell development. Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2331-6
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6794055
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Gene symbol:
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Tial1
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Gene name:
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Tia1 cytotoxic granule-associated RNA binding protein-like 1
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Antibody:
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anti-TIAR 3E6
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
5A:
embryonic day 14.5
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: germ cell of testis |
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Strong |
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5A
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Highly expressed in nuclei of primordial germ cells contained within seminiferous tubules.
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| TS22: Sertoli cell |
Sertoli cell |
Trace |
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5A
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Little or no expression in precursor Sertoli cells lining seminiferous tubules.
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| TS22: Leydig cell |
Leydig cell |
Trace |
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5A
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Relatively low levels of expression in precursor Leydig cells between seminiferous tubules, similar to those found in many tissues throughout the embryos.
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Tial1tm1Mst/Tial1tm1Mst
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Age:
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embryonic day 14.5
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Sex:
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Male
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
5D:
embryonic day 12.5
(more )
Specimen
5D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: primordial germ cell |
Present |
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5D
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Authors state that expression in primordial germ cells was not stage-specific.
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Analysis Tools