About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:175228 Medeiros LA, et al., Mir-290-295 deficiency in mice results in partially penetrant embryonic lethality and germ cell defects. Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14163-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6790207
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: anti-MVH
Assay notes: Fixative was either 4% paraformaldehyde or Bouin's solution.
Results
Specimen 5A: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: germ cell of ovary Present 5A
Specimen 5B: embryonic day 13.5; Mirc5tm1Jae/Mirc5tm1Jae (more )
Structure Level Pattern Image Note
TS21: germ cell of ovary Present 5B In this mutant there is a dramatic reduction in the number positive cells compared to control embryos.
Specimen 5C: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: germ cell of testis Present 5C
Specimen 5D: embryonic day 13.5; Mirc5tm1Jae/Mirc5tm1Jae (more )
Structure Level Pattern Image Note
TS21: germ cell of testis Present 5D In this mutant there is a dramatic reduction in the number positive cells compared to control embryos.
Specimen 5F: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 5F
Specimen 5G: embryonic day 11.5; Mirc5tm1Jae/Mirc5tm1Jae (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 5G Most mutant genital ridges exhibited less than 5% as many germ cells as found in wild-type littermate controls.
Specimen 5H: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 5H
Specimen 5I: embryonic day 11.5; Mirc5tm1Jae/Mirc5tm1Jae (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 5I Most mutant genital ridges exhibited less than 5% as many germ cells as found in wild-type littermate controls.
Specimen S1C: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: oocyte Present S1C
Specimen S1D: postnatal day 5; Mirc5tm1Jae/Mirc5tm1Jae (more )
Structure Level Pattern Image Note
TS28: oocyte Present S1D This mutant contained less than 20% as many oocytes as wild-type ovaries. The number of follicles and the primordial follicle pool are reduced in this mutant compared to wild-type.
Specimen S1G: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: oocyte Present S1G
Specimen S1H: postnatal day 10; Mirc5tm1Jae/Mirc5tm1Jae (more )
Structure Level Pattern Image Note
TS28: oocyte Present S1H The number of follicles and the primordial follicle pool are reduced in this mutant compared to wild-type.
Specimen S1K: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present S1K
Specimen S1L: postnatal day 5; Mirc5tm1Jae/Mirc5tm1Jae (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present S1L The number of germ cells is reduced in this mutant compared to controls.
Specimen S1O: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present S1O
Specimen S1P: postnatal day 10; Mirc5tm1Jae/Mirc5tm1Jae (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present S1P In this mutant germ cells continued to proliferate.
Specimen S4A: embryonic day 12.5 (more )
Note: Double labeled: red - Ddx4; green - Pcna (assay MGI:6790234).
Structure Level Pattern Image Note
TS20: germ cell of ovary Present S4B , S4A , S4C
Specimen S4D: embryonic day 12.5; Mirc5tm1Jae/Mirc5tm1Jae (more )
Note: Double labeled: red - Ddx4; green - Pcna (assay MGI:6790234).
Structure Level Pattern Image Note
TS20: germ cell of ovary Present S4E , S4F , S4D
Specimen S4G: embryonic day 12.5 (more )
Note: Double labeled: red - Ddx4; green - Pcna (assay MGI:6790234).
Structure Level Pattern Image Note
TS20: germ cell of testis Present S4I , S4H , S4G
Specimen S4J: embryonic day 12.5; Mirc5tm1Jae/Mirc5tm1Jae (more )
Note: Double labeled: red - Ddx4; green - Pcna (assay MGI:6790234).
Structure Level Pattern Image Note
TS20: germ cell of testis Present S4L , S4K , S4J
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory