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Gene Expression Data
Assay Details
Assay
Reference: J:312000 Bell BJ, et al., A clock-driven neural network critical for arousal. bioRxiv. 2020;
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6782859
Gene symbol: Ankfn1
Gene name: ankyrin-repeat and fibronectin type III domain containing 1
Reporter: Cre
Antibody: anti-cre (Millipore; MAB3120)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 4a: postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1+ (more )
Note: Double labeled: blue - Ankfn1 reporter (Cre); red - Ankfn1 reporter (tdTomato; assay MGI:6782852).
Structure Level Pattern Image Note
TS28: basal forebrain Present 4a Expression is seen in neuronal cell bodies and projections.
Specimen 4b: postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1+ (more )
Note: Double labeled: blue - Ankfn1 reporter (Cre); red - Ankfn1 reporter (tdTomato; assay MGI:6782852).
Structure Level Pattern Image Note
TS28: lateral hypothalamic area Present 4b Expression is seen in neuronal cell bodies and projections.
TS28: tuberomammillary nucleus Present 4b Expression is seen in neuronal cell bodies and projections.
Specimen 4c: postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1+ (more )
Note: Double labeled: blue - Ankfn1 reporter (Cre); red - Ankfn1 reporter (tdTomato; assay MGI:6782852).
Structure Level Pattern Image Note
TS28: midbrain tegmentum Present Regionally restricted 4c Expression is seen in neuronal cell bodies and projections adjacent to the dorsal raphe.
Specimen 4d: postnatal month 2-4; Ankfn1tm2.1(cre)Mnw/Ankfn1+ (more )
Note: Double labeled: blue - Ankfn1 reporter (Cre); red - Ankfn1 reporter (tdTomato; assay MGI:6782852).
Structure Level Pattern Image Note
TS28: pons Present Regionally restricted 4d Expression is seen in neuronal cell bodies and projections adjacent to the locus coeruleus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/06/2026
MGI 6.24 		The Jackson Laboratory