Summary | Mutation origin | Mutation description | Expression | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Ankfn1tm2.1(cre)Mnw
Name:	ankyrin-repeat and fibronectin type III domain containing 1; targeted mutation 2.1, Mark N Wu
MGI ID:	MGI:6782844
Synonyms:	mWAKECre
Gene:	Ankfn1  Location: Chr11:89280918-89668727 bp, - strand  Genetic Position: Chr11, 54.34 cM
Alliance:	Ankfn1tm2.1(cre)Mnw page

Germline Transmission:	Earliest citation of germline transmission: J:312000
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	129S/SvEv x C57BL/6

Allele Type:    Targeted (Null/knockout, Recombinase, Reporter) Mutation:    Insertion   Ankfn1tm2.1(cre)Mnw expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Ankfn1 (MGI:2686021)   Mutation details: Using homologous recombination in ES cells, the knock-in vector targeted exon 5 of the Ankfn1 locus and was integrated 21 bp into exon 5, replacing the remainder of the exon with a tdTomato-P2A-split Cre-Neo-WPREBGHpA cassette, which causes frameshifted nonsense mutations downstream, resulting in the loss-of-function allele. Neomycin was excised via FLP mice. (J:312000)

Activity:	Tissue activity of this recombinase allele
Driver:	Ankfn1 (mouse) Summary of all recombinase alleles driven by Ankfn1. Comparative matrix view of recombinase activities Alliance mouse Ankfn1 gene page

In Mice Carrying this Mutation:

38 assay results 1 RNA-Seq or microarray experiment(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Ankfn1 Mutation:	48 strains or lines available

Original:	J:312000 Bell BJ, et al., A clock-driven neural network critical for arousal. bioRxiv. 2020;
All:	2 reference(s)