Tg(Casp8*D387A,GFP)#Wll
Transgene Detail
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| Symbol: |
Tg(Casp8*D387A,GFP)#Wll |
| Name: |
transgene insertion, David Wallach |
| MGI ID: |
MGI:6781653 |
| Synonyms: |
BAC-D387A |
| Transgene: |
Tg(Casp8*D387A,GFP)#Wll Location: unknown
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| Alliance: |
Tg(Casp8*D387A,GFP)#Wll page
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| Strain of Origin: |
Not Applicable
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| Transgene Type: |
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Transgenic (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter) |
| Mutation: |
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Insertion
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Mutation details: The transgene insert consists of BAC RP24-238B22, containing the Casp8 gene, which was modified as follows: phage T7 epitope tag coding sequence was inserted upstream of the Casp8 stop codon and an FRT site, an IRES, the GFP green fluorescent protein gene and a second FRT site, were inserted downstream of the stop codon. A twenty nucleotide sequence in intron 1 was replaced with a different sequence of equal length and composition to improve genotyping. Aspartic acid codon 387 was mutated to an alanine codon (p.D387A) to create a noncleavable version of the encoded peptide.
(J:140185)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:140185 Kang TB, et al., Mutation of a self-processing site in caspase-8 compromises its apoptotic but not its nonapoptotic functions in bacterial artificial chromosome-transgenic mice. J Immunol. 2008 Aug 15;181(4):2522-32 |
| All: |
2 reference(s) |
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Analysis Tools
