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Gene Expression Data
Assay Details
Assay
Reference: J:306194 Ghimire S, et al., PEG3 controls lipogenesis through ACLY. PLoS One. 2021;16(5):e0252354
Assay type: RT-PCR
MGI Accession ID: MGI:6780737
Gene symbol: Fasn
Gene name: fatty acid synthase
Probe: Fasn-pE, Fasn-pF
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR was used to obtain these results; Gapdh was used to normalize expression levels.
Results
Image: 5A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
WT-F1 P adult TS28: mammary gland Present Not Specified; total RNA involves: C57BL/6J * C57BL/6N Female
CoKO-F1 P adult TS28: mammary gland Present (a) Not Specified; total RNA involves: C57BL/6J * C57BL/6N Peg3tm1a(EUCOMM)Hmgu/Peg3+Female gene dosage of Peg3 is almost zero
WT-F2 P adult TS28: mammary gland Present Not Specified; total RNA involves: C57BL/6J * C57BL/6N Female
CoKO-F2 P adult TS28: mammary gland Present (b) Not Specified; total RNA involves: C57BL/6J * C57BL/6N Peg3tm1a(EUCOMM)Hmgu/Peg3+Female gene dosage of Peg3 is almost zero
WT-M1 P adult TS28: mammary gland Present Not Specified; total RNA involves: C57BL/6J * C57BL/6N Male
CoKO-M1 P adult TS28: mammary gland Present (c) Not Specified; total RNA involves: C57BL/6J * C57BL/6N Peg3tm1a(EUCOMM)Hmgu/Peg3+Male gene dosage of Peg3 is almost zero
WT-M2 P adult TS28: mammary gland Present Not Specified; total RNA involves: C57BL/6J * C57BL/6N Male
CoKO-M2 P adult TS28: mammary gland Present (d) Not Specified; total RNA involves: C57BL/6J * C57BL/6N Peg3tm1a(EUCOMM)Hmgu/Peg3+Male gene dosage of Peg3 is almost zero
Notes:
(a) Expression levels are increased 3.7 fold in the mutant.
(b) Expression levels are increased 3.5 fold in the mutant.
(c) Expression levels are reduced 80% in the mutant.
(d) Expression levels are reduced 70% in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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