Myod1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:309563 Rafipay A, et al., Knockdown of slit signaling during limb development leads to a reduction in humerus length. Dev Dyn. 2021 Sep;250(9):1340-1357
Assay type:	RNA in situ
MGI Accession ID:	MGI:6780209
Gene symbol:	Myod1
Gene name:	myogenic differentiation 1

Probe:	Myod1 probe19
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 2C Myod1 Slit1+/+: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: upper leg	Present		2C Myod1 Slit1+/+
TS23: foot	Present	Regionally restricted	2C Myod1 Slit1+/+	Expression in footplate proximal to digits.

Specimen 2C Myod1 Slit1-/- Slit2-/-: embryonic day 15.5; Slit1^tm1.1Matl/Slit1^tm1.1Matl, Slit2^tm1Matl/Slit2^tm1Matl (more

)

Structure	Level	Pattern	Image	Note
TS23: upper leg	Present		2C Myod1 Slit1-/- Slit2-/-
TS23: foot	Present	Regionally restricted	2C Myod1 Slit1-/- Slit2-/-	Expression in footplate proximal to digits.

Specimen 4B Myod1 Slit3+/+: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: hand	Present	Regionally restricted	4B Myod1 Slit3+/+	Expression in proximal limb mesenchyme and handplate
TS23: upper arm	Present	Regionally restricted	4B Myod1 Slit3+/+	Expression in proximal limb mesenchyme and handplate
TS23: forelimb mesenchyme	Present	Regionally restricted	4B Myod1 Slit3+/+	Expression in a central band of expression connecting expressing regions.

Specimen 4B Myod1 Slit3-/-: embryonic day 15.5; Slit3^tm1.1Dor/Slit3^tm1.1Dor (more

)

Structure	Level	Pattern	Image	Note
TS23: hand	Present	Regionally restricted	4B Myod1 Slit3-/-	Expression in proximal limb mesenchyme and handplate
TS23: upper arm	Present	Regionally restricted	4B Myod1 Slit3-/-	Expression in proximal limb mesenchyme and handplate
TS23: forelimb mesenchyme	Present	Regionally restricted	4B Myod1 Slit3-/-	Expression in a central band of expression connecting expressing regions.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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