Myod1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:309563 Rafipay A, et al., Knockdown of slit signaling during limb development leads to a reduction in humerus length. Dev Dyn. 2021 Sep;250(9):1340-1357
Assay type:	RNA in situ
MGI Accession ID:	MGI:6780209
Gene symbol:	Myod1
Gene name:	myogenic differentiation 1

Probe:	Myod1 probe19
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 2C Myod1 Slit1+/+: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: upper leg	Present		2C Myod1 Slit1+/+
TS23: foot	Present	Regionally restricted	2C Myod1 Slit1+/+	Expression in footplate proximal to digits.

Specimen 2C Myod1 Slit1-/- Slit2-/-: embryonic day 15.5; Slit1^tm1.1Matl/Slit1^tm1.1Matl, Slit2^tm1Matl/Slit2^tm1Matl (more

)

Structure	Level	Pattern	Image	Note
TS23: upper leg	Present		2C Myod1 Slit1-/- Slit2-/-
TS23: foot	Present	Regionally restricted	2C Myod1 Slit1-/- Slit2-/-	Expression in footplate proximal to digits.

Specimen 4B Myod1 Slit3+/+: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: hand	Present	Regionally restricted	4B Myod1 Slit3+/+	Expression in proximal limb mesenchyme and handplate
TS23: upper arm	Present	Regionally restricted	4B Myod1 Slit3+/+	Expression in proximal limb mesenchyme and handplate
TS23: forelimb mesenchyme	Present	Regionally restricted	4B Myod1 Slit3+/+	Expression in a central band of expression connecting expressing regions.

Specimen 4B Myod1 Slit3-/-: embryonic day 15.5; Slit3^tm1.1Dor/Slit3^tm1.1Dor (more

)

Structure	Level	Pattern	Image	Note
TS23: hand	Present	Regionally restricted	4B Myod1 Slit3-/-	Expression in proximal limb mesenchyme and handplate
TS23: upper arm	Present	Regionally restricted	4B Myod1 Slit3-/-	Expression in proximal limb mesenchyme and handplate
TS23: forelimb mesenchyme	Present	Regionally restricted	4B Myod1 Slit3-/-	Expression in a central band of expression connecting expressing regions.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/14/2024 MGI 6.23