Cdh5 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:309563 Rafipay A, et al., Knockdown of slit signaling during limb development leads to a reduction in humerus length. Dev Dyn. 2021 Sep;250(9):1340-1357
Assay type:	RNA in situ
MGI Accession ID:	MGI:6780207
Gene symbol:	Cdh5
Gene name:	cadherin 5

Probe:	Cdh5 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 2C Cdh5 Slit1+/+: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: forelimb mesenchyme	Present	Regionally restricted	2C Cdh5 Slit1+/+	Expression throughout limb mesenchyme.
TS23: forelimb interdigital region mesenchyme	Present	Regionally restricted	2C Cdh5 Slit1+/+	Expression up to proximal interphalangeal joint along digits. More intense regions of expression interdigitally and at digit base.
TS23: forelimb digit mesenchyme	Present	Regionally restricted	2C Cdh5 Slit1+/+	No expression in avascular region at digit tips.

Specimen 2C Cdh5 Slit1-/- Slit2-/-: embryonic day 15.5; Slit1^tm1.1Matl/Slit1^tm1.1Matl, Slit2^tm1Matl/Slit2^tm1Matl (more

)

Structure	Level	Pattern	Image	Note
TS23: hindlimb mesenchyme	Present	Regionally restricted	2C Cdh5 Slit1-/- Slit2-/-	Expression throughout limb mesenchyme.
TS23: hindlimb interdigital region mesenchyme	Present	Regionally restricted	2C Cdh5 Slit1-/- Slit2-/-	Expression up to proximal interphalangeal joint along digits. More intense regions of expression interdigitally and at digit base.
TS23: hindlimb digit mesenchyme	Present	Regionally restricted	2C Cdh5 Slit1-/- Slit2-/-	No expression in avascular region at digit tips.

Specimen 4B Cdh5 dorsal Slit3+/+: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: hand	Present	Regionally restricted	4B Cdh5 dorsal Slit3+/+	Expression through handplate.
TS23: forelimb digit mesenchyme	Strong	Regionally restricted	4B Cdh5 dorsal Slit3+/+	Strong expression along digit borders.

Specimen 4B Cdh5 dorsal Slit3-/-: embryonic day 15.5; Slit3^tm1.1Dor/Slit3^tm1.1Dor (more

)

Structure	Level	Pattern	Image	Note
TS23: hand	Present	Regionally restricted	4B Cdh5 dorsal Slit3-/-	Expression through handplate.
TS23: forelimb digit mesenchyme	Strong	Regionally restricted	4B Cdh5 dorsal Slit3-/-	Strong expression along digit borders.

Specimen 4B Cdh5 ventral Slit3+/+: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: hand	Present	Regionally restricted	4B Cdh5 ventral Slit3+/+	Expression through handplate.
TS23: forelimb digit mesenchyme	Strong	Regionally restricted	4B Cdh5 ventral Slit3+/+	Strong expression along digit borders.

Specimen 4B Cdh5 ventral Slit3-/-: embryonic day 15.5; Slit3^tm1.1Dor/Slit3^tm1.1Dor (more

)

Structure	Level	Pattern	Image	Note
TS23: hand	Present	Regionally restricted	4B Cdh5 ventral Slit3-/-	Expression through handplate.
TS23: forelimb digit mesenchyme	Strong	Regionally restricted	4B Cdh5 ventral Slit3-/-	Strong expression along digit borders.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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