Gene Expression Data
Assay Details
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Assay
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Reference:
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J:112970
Ortiz-Alvarado R, et al., Expression of tryptophan hydroxylase in developing mouse taste papillae. FEBS Lett. 2006 Oct 2;580(22):5371-6
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6771692
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Gene symbol:
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Tph2
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Gene name:
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tryptophan hydroxylase 2
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Antibody:
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anti-TPH (WH-3)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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This antibody recognizes both Tph1 and Tph2.
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Results
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Specimen
2A:
embryonic day 15.0
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: tongue mesenchyme |
Very strong |
Regionally restricted |
2A
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Expression is in fibers just below the developing papillae epithelium.
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Specimen
2B:
embryonic day 17.0
(more )
Specimen
2B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 17.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: tongue mesenchyme |
Very strong |
Regionally restricted |
2B
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Expression is in fibers just below the developing papillae epithelium.
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Specimen
2C:
postnatal day 0
(more )
Specimen
2C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: circumvallate papillae |
Present |
Regionally restricted |
2C
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Expression is in the nerve plexus within the papillae. Exression is absent in the dorsal epithelium.
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| TS27: nerve |
Present |
Regionally restricted |
2C
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Expression is in the nerve plexus within the papillae. Exression is absent in the dorsal epithelium.
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| TS27: tongue |
Present |
Regionally restricted |
2C
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Expression is in the nerve fibers surrounding the papillae trench wall epithelium.
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Specimen
3A:
postnatal day 11
(more )
Note: Double labeled: green - Tph1 and Tph2; red - Gnat3 (assay MGI:6771675).
Specimen
3A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 11
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Tph1 and Tph2; red - Gnat3 (assay MGI:6771675).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: circumvallate papillae |
Present |
Regionally restricted |
3F
,
3C
,
3D
,
3A
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Expression is in the central nerve plexus within the papillae.
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| TS28: nerve |
Present |
Regionally restricted |
3F
,
3C
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3D
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3A
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Expression is in the central nerve plexus within the papillae.
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| TS28: taste bud |
Present |
Single cells |
3F
,
3A
,
3D
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3C
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Analysis Tools